1,25-二羟维生素D_3联合塞莱昔布对胃癌细胞株SGC-7901凋亡的影响  

Effects of 1,25-dihydroxyvitamin D_3 and Celebrex on Aapoptosis of Gastric Cancer Cell Line SGC-7901

作　　者：彭昌能[1] 李国庆[1] 谷苗[2] 

机构地区：[1]湖南省郴州市第一人民医院消化内科,423000 [2]南华大学附属第二医院消化内科

出　　处：《医学研究杂志》2014年第1期74-78,共5页Journal of Medical Research

摘　　要：目的本研究探讨1,25-二羟维生素D3(1 alpha,25-dihydroxy vitamin D3,VitD3)联合塞莱昔布(CELE)对胃癌细胞株SGC-7901凋亡诱导作用及Bcl-2、Bax蛋白表达的影响,探讨其联合应用是否具有协同抗胃癌作用,并初步探讨ViD3与CELE联合应用抗胃癌的可能作用机制,为临床制定合理的联合化疗方案,寻求最佳治疗效果提供理论依据。方法体外培养胃癌细胞SGC-7901至指数生长期,加入一定浓度的CELE、VitD3及CELE和VitD3,作用48h后,采用吖啶橙染色观察细胞凋亡,Western blot法检测Bcl-2、Bax蛋白的表达变化,分析两种药物对胃癌细胞SGC-7901的增殖、凋亡及相关基因表达的影响。结果吖啶橙染色观察细胞凋亡从形态学上证实了VitD3和CELE对胃癌细胞的诱导凋亡作用,且两药联合增强诱导凋亡作用;VitD3、CELE及二者联合应用均显著抑制SGC-7901细胞Bcl-2蛋白的表达、促进Bax蛋白的表达(P<0.05),联合用药组与单一用药组相比差异有统计学意义(P<0.05)。结论 VitD3和CELE可诱导胃癌SGC-7901细胞凋亡,其凋亡机制之一可能为通过抑制Bcl-2、并促进Bax的表达,从而改善Bcl-2/Bax的平衡实现的。两者联合应用对胃癌细胞SGC-7901诱导凋亡具有协同作用。Objective This study explores the 1,25 -dihydroxyvitamin D3 combined with celecoxib （CELE） on apoptosis and ex- pression of Bcl - 2, Bax protein in gastric cancer cell line SGC - 7901 and to explore whether the combination of synergistic anti - cancer effect and to explore the possible mechanism of the combined application of VitD3 and CELE against gastric cancer. It is seeking to provide a theoretical basis for optimal therapeutic effect for the clinical development of rational combination chemotherapy. Methods To culture gastric cancer cells SGC - 7901 in vitro to the exponential growth phase, adding a certain concentration CELE, VitD3 and CELE and VitD3.48 h after, acridine orange staining was used to observe the cell apoptosis and Western blot was used to detect the expression of Bcl -2, Bax protein. The effects of two drugs on gastric cancer SGC -7901 cells apoptosis and related gene expression were analyzed. Re- sults Apoptotic cells were observed in morphology confirmed the effect of apoptosis induced by VitD3 and CELE in gastric carcinoma cells by acridine orange staining, and the two drugs combined with enhanced induction of apoptosis; VitD3. CELE and the combination of the two methods were significantly inhibited SGC -7901 cell expression of Bcl -2, enhance the expression of Bax protein （P 〈 0.05 ） , a sig- nificant drug combination group and monotherapy group compared to the difference （P 〈 0.05 ）. Conclusion VitD3 and CELE can induce the apoptosis of gastric cancer SGC -7901 ceils： one of the mechanisms of apoptosis may be expressed through inhibition of Bcl -2, and promote Bax, so as to improve the balance of Bcl - 2/Bax implementation. The combination of the two has a synergistic effect on gastric cancer cell apoptosis induced by SGC -7901.

关 键 词：VITD3 CELE 胃癌 凋亡 

分 类 号：R735[医药卫生—肿瘤]