欧美杂种山杨微扦插不定根发生过程的解剖学研究  

Morphological and Anatomical Features during the Formation of Adventitious Roots of Populus tremula×P.tremuloides by Micro-cuttage Multiplication

作　　者：周容涛[1] 刘洋[1] 冷珊珊[1] 王娜[1] 闫绍鹏[1] 

机构地区：[1]东北林业大学生命科学学院,哈尔滨150040

出　　处：《西北植物学报》2013年第11期2184-2188,共5页Acta Botanica Boreali-Occidentalia Sinica

基　　金：林木遗传育种国家重点实验室(东北林业大学)开放基金项目(201106);东北林业大学大学生创新训练项目(201210225113)

摘　　要：采用石蜡切片技术,以欧美杂种山杨插穗基部茎段为实验材料,连续解剖观察插穗不定根发生发育过程,分析根原基发生部位与扦插生根的关系。结果显示:欧美杂种山杨插穗不定根的发生过程分为4个时期,为根原基诱导期,不定根起始期、表达期和伸长生长期。根原基诱导期维管形成层产生具有分生组织特点的薄壁细胞;不定根起始期,维管形成层及附近的薄壁细胞脱分化,形成不定根原基发端细胞;不定根表达期,根原基发端细胞不断分裂成具有方向性的根原基,根原基穿过韧皮射线和皮层,向皮孔方向发展;不定根伸长生长期,根原基从皮孔伸出,其内部的维管系统开始发育,形成不定根。研究认为,欧美杂种山杨为皮部诱导生根类型,不定根原基起源于维管形成层区,起源部位单一,扦插难生根。Morphological and anatomical features were observed during the formation of adventitious roots of Populus tremula X P. tremuloides by micro-cuttage multiplication with paraffin method, and the relationship between root primordia site and rooting was also analyzed. The results showed that ontogeny process of adventitious roots can be divided into four stages. The stage of adventitious root primordium induction. the cells of vascular cambium divided into parenchyma cell groups which contained the characteristics of meristem. The stage of adventitious root origination.the vascular cambium cell kept dividing and form adventitious root primordium initial cells which showed strong mitogenic ability. The stage of adventitious root expression, the adventitious root primordium initial cells kept dividing and formed root primordium with directivity. The stage of adventitious root elongation growth.the root primordium stretched out from lenticel and nick,inner vascular system well matured, then the adventitious root formed. The study showed that Populus tremula X P. tremuloides was bark inductive root formation type,adventitious root primordia originated in the vascular cambium area,single site of origin,was hard rooting type.

关 键 词：欧美杂种山杨 扦插 解剖学 不定根 

分 类 号：Q944.54[生物学—植物学]