厚朴汁炙远志含药血清对胃Cajal间质细胞活力及nNOS表达的影响  

Study on the effects of the radix Polygalae decoction processed by Magnolia officinalis medicated serum on the expression of nNOS of interstitial cells of cajal in vitro

作　　者：唐丹霞[1] 王建[1] 袁奕[1] 郑新光[1] 张璐[1] 

机构地区：[1]成都中医药大学药学院中药教研室,成都610075

出　　处：《中药药理与临床》2013年第6期100-103,共4页Pharmacology and Clinics of Chinese Materia Medica

基　　金：国家自然科学基金(NO:81173567)

摘　　要：目的:考察厚朴汁炙远志炮制品含药血清对胃Cajal间质细胞(interstitial cells of Cajal,ICC)活力及胞内nNOS表达的影响,从细胞水平阐释其降低胃动力障碍的生物学机制。方法:采用体外培养胃Cajal间质细胞,再加入含药血清与之共培养的方法开展实验。实验分为空白组、厚朴10 g/kg组、远志20 g/kg组、厚朴汁炙远志炮制品高剂量30 g/kg组4个不同组,采用MTT比色法检测细胞活性、Western-Blot法分析各药对细胞内nNOS表达的影响。结果:远志20 g/kg组ICC细胞的OD值明显低于空白组,且细胞突起变短,网状脱落;而厚朴10 g/kg组、厚朴汁炙远志30 g/kg组细胞OD值明显高于空白组和单味远志组。免疫印迹法检测结果显示,远志20 g/kg组的nNOS的表达与空白组比较明显增强,厚朴10 g/kg组的表达明显下降,远志20 g/kg组呈下降趋势。结论:远志能抑制胃动力起搏细胞活性,抑制胃间质细胞nNOS表达增强;而厚朴汁炙远志能明显促进细胞活性、下调nNOS表达从而缓解胃动力障碍。Objective： To study the Polygalae decoction processed by Magnolia officinalis medicated production on cultured gastric interstitial cells of Cajal （ interstitial ceils of Cajal, ICC） of the expression of nNOS to explain the cellular mechanisms of NO gastrointestinal dysfunction i. Methods： ICCs were cultured in vitro, then adding method containing serum and co culture experiments. The experiment was divided into blank group, Magnolia 10 g/kg group, Polygala 20 g/kg group, Polygalae decoction processed by Magnolia 30g/kg group such four different groups, using the MTT colorimetric detection cell activity, Western-Blot method of intracellular the expression of nNOS analysis. Results： Compared with blank group, Polygala 20 g/kg group of cells＇OD values was significantly lower （P 〈 0.05, P 〈 0.01 ） , and shorter cell processes, reticular off; while Magnolia 10 g/kg group, Polygala decoction processed by Magnolia products high-dose 30 g/kg group cells OD values were significantly higher（ P 〈 0.05, P 〈 0.01 ）. Western blot showed that compared with the blank group, the expression of nNOS of Polygala group was significantly enhanced, Magnolia 10 g/kg group was significantly decreased, Polygala decoction processed by Magnolia 30 g/kg group declined. Conclusion： Polygalae can inhibit tile activity of ICC motility, and the expression of nNOS; and Polygalae decoction processed by Magnolia can obviously promote the down-regulation of nNOS expression in order to ease the cell activity, gastric motility disorders.

关 键 词：远志 厚朴汁炙远志 含药血清 胃间质细胞 NNOS 

分 类 号：R285[医药卫生—中药学]