石河子垦区犬瘟热病毒流行株H基因遗传变异分析  

Genetic Variation of H Gene of Canine Distemper Virus from Shihezi Area

作　　者：王国超[1] 赵春光[1] 乔军[1] 孟庆玲[1] 陈双庆[1] 陈创夫[1] 

机构地区：[1]石河子大学动物科技学院,预防兽医学新疆自治区重点实验室,新疆石河子832003

出　　处：《西北农业学报》2013年第12期192-196,共5页Acta Agriculturae Boreali-occidentalis Sinica

基　　金：兵团国际科技合作计划(2012BC006);农业公益行业专项子课题(201303037-5)

摘　　要：为了解石河子垦区的CDV流行株H基因遗传变异特点，设计2对特异性引物，通过RTPCR方法对29份临床疑似犬瘟热的病料进行检测，对于检测CDV阳性的病料进行流行株H基因克隆、测序和序列分析，结果检测出7份阳性病料。序列分析发现，7个流行株H基因之间的同源率在91．0％～98．9％，与疫苗株（FJ461699．1）的同源率为86．9％～91．7％，存在一定的变异。糖基化位点及抗原表位预测分析发现，与参考株Onderstepoort相比，7个流行株推导的H蛋白糖基化位点、抗原表位均有不同程度的改变。遗传进化分析显示，CDVshzCDVshz6亲缘关系较近，它们与CDVshz7相距较远。研究结果提示，石河子垦区发病犬中存在cDV变异株。In order to understand the genetic variation of Canine distemper virus （CDV） in the Shihezi area, 29 clinically suspected infection CDV samples were detected by RT-PCR method with two pairs of specific primers designed on H gene, and positive samples were conducted cloning, sequencing and sequence analysis. The results showed that seven positive samples were detected. The sequence of H gene in these seven epidemic strains, with the homologous rate range from 91.0% to 98.9% ,had the homologous rate ranging from 86.9%to 91.7% with the vaccine isolate strains （FJ461699.1）, and there were a certain variation. Potential glycosylation sites and antigenic epitopes of the epidemic strains H genes analysis results indicated the seven epidemic strains H genes varied on glycosylation sites, antigenic epitopes compared with the reference strain Onderstepoort. Phylogenetic analysis showed that CDVshzl to CDVshz6 with a close relationship were far from CDVshzT. The results sug gested that there are CDV variant strains existed in diseased dogs in Shihezi area.

关 键 词：石河子垦区 犬瘟热病毒 H基因 遗传变异 

分 类 号：S852.65[农业科学—基础兽医学]