Effects of rice straw on the cell viability, photosynthesis, and growth of Microcystis aeruginosa  

作　　者：苏文 Johannes A.HAGSTR?M 贾育红 卢亚萍 孔繁翔 

机构地区：[1]State Key Laboratory of Lake & Environment Science,Nanjing Institute of Geography and Limnology,Chinese Academy of Sciences [2]University of Chinese Academy of Sciences [3]Ecology and Evolution in Microbial Model Systems,EEMiS,School of Natural Sciences,Linnaeus University

出　　处：《Chinese Journal of Oceanology and Limnology》2014年第1期120-129,共10页中国海洋湖沼学报（英文版）

基　　金：Supported by the National Basic Research Program of China(973 Program)(No.2008CB418002);the National Major Science and Technology Program for Water Pollution Control and Treatment(No.2012ZX07103-002);the CAS/SAFEA International Partnership Program for Creative Research Teams of Chinese Academy of Sciences(No.KZZDEW-TZ-08-01);the National Natural Science Foundation of China(No.20807043)

摘　　要：Rice straw is supposed to be an environment-friendly biomaterial for inhibiting the growth of harmful blooms of the cyanobacterium Microcystis aeruginosa. However, its potential mechanism is not well known. To explore this mechanism, the growth, cell viability(esterase activity, membrane potential, and membrane integrity), photosynthesis, and cell size of M. aeruginosa were determined using fl ow cytometry and Phyto-PAM after exposure to rice straw extracts(RSE). The results show that doses from 2.0 to 10.0 g/L of RSE effi ciently inhibited the alga for 15 days, while the physiologic and morphologic responses of the cyanobacteria were time-dependent. RSE interfered with the cell membrane potential, cell size, and in vivo chlorophyll- a fl uorescence on the fi rst day. After 7 days of exposure, RSE was transported into the cytosol, which disrupted enzyme activity and photosynthesis. The cyanobacteria then started to repair its physiology(enzyme activity, photosynthesis) and remained viable, suggesting that rice straw act as an algistatic agent.Rice straw is supposed to be an environment-friendly biomaterial for inhibiting the growth of harmful blooms of the cyanobacterium Microcystis aeruginosa. However, its potential mechanism is not well known. To explore this mechanism, the growth, cell viability （esterase activity, membrane potential, and membrane integrity）, photosynthesis, and cell size ofM. aeruginosa were determined using flow cytometry and Phyto-PAM after exposure to rice straw extracts （RSE）. The results show that doses from 2.0 to 10.0 g/L of RSE efficiently inhibited the alga for 15 days, while the physiologic and morphologic responses of the cyanobacteria were time-dependent. RSE interfered with the cell membrane potential, cell size, and in vivo chlorophyll-a fluorescence on the first day. After 7 days of exposure, RSE was transported into the cytosol, which disrupted enzyme activity and photosynthesis. The cyanobacteria then started to repair its physiology （enzyme activity, photosynthesis） and remained viable, suggesting that rice straw act as an algistatic agent.

关 键 词：esterase activity membrane potential Phyto-PAM rice straw MICROCYSTIS flow cytometry 

分 类 号：X173[环境科学与工程—环境科学]