响应面法优化镰刀霉菌JN158产色素培养基  

Optimization of Medium Composition for Pigment Production by Fusarium sp. JN158 with Response Surface Methodology

作　　者：王永威[1] 廖祥儒[1] 黎循航[1] 蔡宇杰[1] 

机构地区：[1]江南大学生物工程学院,工业生物技术教育部重点实验室,江苏无锡214122

出　　处：《食品科学》2014年第1期120-124,共5页Food Science

基　　金：国家自然科学基金项目(21045007);江苏省科技创新与成果转化(重大科技支撑与自主创新)专项(BY2010117)

摘　　要：用Plackett-Burman试验设计和响应面法(response surface methodology,RSM)对镰刀霉菌JN158产色素的发酵培养基7种营养物质配比进行优化。结果表明:蔗糖、花生饼粉和FeSO4·7H2O的添加量显著影响色素产量;用最陡爬坡试验使这3个显著因素的添加量接近最大响应区域,优化后,镰刀霉菌产色素的培养基添加量分别为:蔗糖30.18 g/L、花生饼粉4.86 g/L、K2HPO4·3H2O 1 g/L、NaCl 0.5 g/L、MgSO4·7H2O 0.5 g/L、CaCl20.5 g/L、FeSO4·7H2O 0.0122 g/L,预测的最大色素产量是1.268 g/L,验证实验得到的色素产量为(1.252±0.011)g/L,与预测值相近,优化后色素的产量是以前的2.9倍。Plackett-Burman design and response surface methodology （RSM） were applied to optimize 7 components in the medium for pigment production by Fusarium sp.JN158. The results showed that the yield of pigment was mainly influenced by the concentrations of glucose, peanut meal and FeSO4·7H2O. With central composite design and RSM, the optimal medium composition and concentrations was determined to be sucrose （30.18 g/L）, peanut meal （4.86 g/L）, K2HPO4·3H2O（1 g/L）,NaCl （0.5 g/L）, MgSO4·7H2O （0.5 g/L）, CaCl2 （0.5 g/L）, and FeSO4·7H2O （0.0122 g/L）. The experimental yield of pigment produced by Fusarium sp. JN158 in the optimized medium was （1.252±0.011） g/L, which was close to the predicted value of 1.268 g/L.

关 键 词：镰刀霉菌 色素 PLACKETT-BURMAN 设计 响应面 培养基 

分 类 号：Q81[生物学—生物工程]