精-甘-天冬-丝氨酸肽对兔血管舒张功能及左旋精氨酸/一氧化氮途径的影响  

Effects of Arg-Gly-Asp-Ser peptide on vascular relaxation and L-Arginine/Nitric oxide system in rabbits

作　　者：姚兴海[1] 王晓红 王雯[1] 符民桂 张立克[1] 唐朝枢 

机构地区：[1]首都医科大学病理生理教研室,北京100054 [2]北京医科大学第一临床医学院,北京100034

出　　处：《基础医学与临床》2000年第6期530-533,共4页Basic and Clinical Medicine

摘　　要：本文观察了RGDS肽对兔离体股动脉舒张反应、主动脉一氧化氮(NO)生成、一氧化氮合酶(NOS)活性及左旋精氨酸(L-Arg)转运的影响。结果发现，RGDS肽呈剂量依赖性地引起内皮依赖和非依赖性的血管扩张；增加NOS活性及NO的产生，可被L-NNA所逆转。RGDS肽亦可增加血管组织高和低亲和性L-Arg的最大转运速率(与对照组相比分别增加126%和64%，P＜0.05和P＜0.01)。表明RGDS肽可显著增加L-Arg转运及NOS的活性，对内皮衍化舒张因子(EDRF)/NO系统具有重要调节作用。The present study investigated the effects of Arg-G ly-Asp-Ser(RGDS) Peptide on femoralis relaxation, aortic nitric oxide (NO) pro duction, activity of NO synthase and L-arginine transport of rabbit in vitro . Results showed that RGDS peptide could cause vasorelaxation in the pre sence and absence of a functional endothelium in dose-dependence. When incubat ed With RGDS peptide, aortic slices NOS activity and NO production were obviousl y enhanced, and could be countered after using L-NNA. Furthermore, the maximal velocity of high and low affinity L-Arg transport were promoted about 126% and 64% more than that of control group ( P＜0.05 and P＜ 0.01) by RGDS pep tide. The results suggested RGDS peptide might affect several point of L-Arg/N O system to different extents.

关 键 词：RGDS肽 一氧化氮 NOS 左旋精氨酸 血管扩张 

分 类 号：R341[医药卫生—基础医学]