人胎儿脾NPY,CGRP,SP和VIP神经的分布  

Distribution of NPY , CGRP , SP , VIP nerves in spleen of human fetus

作　　者：陈竺[1] 张远强[2] 蒋尔鹏[2] 补永安[2] 席芳 

机构地区：[1]乌鲁木齐军医学院解剖学教研室,新疆呼图壁831200 [2]第四军医大学基础部组织学与胚胎学教研室,陕西西安710033

出　　处：《第四军医大学学报》2000年第10期1215-1218,共4页Journal of the Fourth Military Medical University

基　　金：新疆军区卫生部资助课题

摘　　要：目的　研究人胎儿脾 NPY,CGRP,SP和 VIP神经的分布和意义 .方法　应用抗 NPY,CGRP,SP和 VIP的ABC免疫组织化学法结合 GDN显色技术研究人胎儿脾肽能神经的定位和分布 .结果　 NPY- IR神经纤维沿脾的动脉、静脉及其分支走行 ,在被膜、小梁、白髓、红髓和边缘区亦有分布 .CGRP- IR和 SP- IR神经纤维在脾脏的分布相同 ,主要沿脾动脉走行 ,中央动脉周围尤为丰富 ,白髓和红髓的淋巴组织中有少许分布 .VIP- IR神经纤维主要分布在中央动脉周围 ,白髓和边缘区有零星分布 .人脾肽能神经纤维末梢与免疫细胞接触密切 ,揭示它们对免疫细胞的发育和活性具有调节作用 .结论　人脾含 NPY,CGRP,SP和 VIP神经的发现 。AIM To study the distribution and significance of NPY , CGRP , SP , VIP containing nerves in the spleen of human fetus. METHODS The localization and distributionof peptidergic nerves in the human fetal spleen were identified with anti NPY, CGRP, SP, VIP immunohistochemical ABC method combined with nickel enhancement technique. RESULTS The nerve fibers containing NPY immunoreactivity were mainly distributed along the branches of arteries and veins. They were also observed in the capsule, in the lymphoid tissues of white pulp, red pulp and marginal zone. Fibers staining for CGRP and SP were completely overlapped indicating coexistence. They were present in perivascular plexus supplying arteries. Some CGRP IR and SP IR fibers traveled between the lymphoid tissue of white pulp and red pulp. VIP IR nerve fibers occurred around the central arterioles. Only very rarely did they course in the lymphoid parenchyma. The positive fibers were closely contacted with immunocytes, which suggested that they might regulate the development and activity of immunocytes. CONCLUSION These findings provide morphological evidence for studies concerning the neuropeptide involved in the regulation of the local spleen immune function.

关 键 词：神经肽 肽能神经 免疫组织化学 胎脾 NPY CGRP 

分 类 号：R329.1[医药卫生—人体解剖和组织胚胎学]