人VEGF基因的克隆及卵巢癌中VEGF基因重排的检测  

Cloning of human VEGF gene and detection of its rearrangement in ovary cancer

作　　者：金明[1] 赵君庸[2] 严瑞兰[3] 惠宏襄[1] 王剑波 王成济[1] 杨安钢[1] 

机构地区：[1]第四军医大学基础部生物化学与分子生物学教研室,陕西西安710033 [2]西安医科大学生物化学教研室 [3]西京医院妇产科 [4]基础部病理学教研室

出　　处：《第四军医大学学报》2000年第10期1298-1300,共3页Journal of the Fourth Military Medical University

基　　金：国家自然科学基金资助项目!(3970 0 14 7)

摘　　要：目的　探讨 VEGF高表达的卵巢癌中 VEGF基因结构有无异常 .方法　用 RT- PCR方法自胎盘中克隆 VEGFc DNA以作为杂交探针 ;收集临床卵巢癌手术切除标本 ,以正常卵巢组织为对照 ,从中提取基因组 DNA并进行 Southern印迹杂交 .结果　序列分析表明所获得基因正确 ,Southernblot分析 10 / 19呈现出大于 2 3 kb片段和 4.3 kb片段的异常片段 ,而不同于正常对照组织的大于 30 kb,6 .5 kb和 2 .5 kb.AIM To study whether there is a difference in the structure of V EGF gene between the normal tissue and the ovarian cancer overexpressing VEGF. METHODS VEGF cDNA was cloned from placenta by RT PCR. The ovarian cancer samples were collected in clinic.With the normal ovariantissue as the control, genomic DNA from the samples was extracted was extracted and compared with the normal ovarian tissue and Southern blotting was done. RESULTS VEGF cDNA clone from placenta was proved to be correct. There are three fragments (>30 kb,6.5 kb,2.5 kb) in normal control,while 10/19 samples only had two (>23kb and 4.3 kb).CONCLUSION The rearrangement of VEGF in the ovary cancer overexpressing VEGF does exist.

关 键 词：卵巢癌 VEGF基因 克隆 基因重排 DNA印迹杂交 

分 类 号：R737.31[医药卫生—肿瘤]