胃癌单克隆抗体的生物学特性  

Biological characterization of monoclonal antibody agsinst gastric cancer cells.

作　　者：吕同德[1] 张辉[1] 高福兴[1] 张银霞[1] 于兰[1] 

机构地区：[1]兰州军区兰州总医院医学实验科,730050

出　　处：《兰州医学院学报》2000年第3期5-7,共3页Journal of Lanzhou Medical College

摘　　要：目的　探索胃癌单克隆抗体的生物学特性。方法　用国内胃癌细胞株免疫小鼠 ,通过杂交瘤技术 ,获得一系列单克隆抗体。结果　经免疫荧光染色 ,按抗原分布特性可分为三种不同类型 :① 1D1 1:只与胃癌细胞株反应 ,不与其它组织细胞反应。② 1A2、5B10、4D6:不但与胃癌细胞株反应 ,也与所有正常、异常组织反应。③ 1D1 2 :与胃癌细胞株反应 ,还与某些癌瘤等组织细胞反应 ,但是不与绝大多数正常组织细胞反应。结论　 1D1 2是一种有研究价值的肿瘤相关抗体。Objective To investigate reactivty of monoclonal antibody(McAb)against gastric cancer.Methods A series of monoclonal antibodies against gastric ancer was produced by hybridomas techniques.Results These antibodies could classified into 3 different groups by immunofluorescence staining.①McAb,GMG1 1D1 1,which was GGC 8140 speific and reacted with no other cell line or tissue,cell samples;②McAb,GMG1 1A2 2,5B10,4D6,which reacted with GGC 8140 and were also reactive with a number of normal and tomour samples;③McAb,GMG1,1d1 2,which reated with GGC 8140 and was also reactire with certain cancer or Non cancer tissue but not with the great majority of normal tissues,It has a remarkable specificity for glanddular epithelial of any tissue whether it be found in cancer or Non cancer.Conclusions These antibodies wer not tumour specific,butGMG1 1D1 2 was Tumour associted antibody.

关 键 词：胃癌 肿瘤相关抗原 单克隆抗体 

分 类 号：R735.2[医药卫生—肿瘤]