肿瘤微血管密度和血管内皮生长因子的表达与胃癌病理及临床分期研究  

Studies on the Relationship between Microvessel Density and Expression of Vascular Endothelial Cell Growth Factor and Pathological Types and Clinical Stages in Gastric Carcinoma

作　　者：陶凯雄[1] 陈道达[1] 田源[1] 杨秀萍[1] 

机构地区：[1]同济医科大学附属协和医院外科,武汉430022

出　　处：《同济医科大学学报》2000年第6期534-536,共3页Acta Universitatis Medicinae Tongji

基　　金：卫生部临床学科重点资助项目(67168)

摘　　要：为研究肿瘤微血管密度（ＭＶＤ）和肿瘤血管内皮生长因子（ＶＥＧＦ）的表达水平与胃癌的病理及临床分期间的关系。应用抗人Ⅷ因子相关抗原抗体，抗ＶＥＧＦ单克隆抗体，采用ＡＢＣ免疫组化法，分别检测４５例胃癌组织中ＭＶＤ和ＶＥＧＦ的表达水平。结果发现，胃癌组织中ＭＶＤ平均值为３１．４±１４．３，ＶＥＧＦ表达阳性率为５３．３％（２４／４５），ＶＥＧＦ表达阳性者 ＭＶＤ值显著高于阴性者（Ｐ＜０．０５）；ＭＶＤ值和 ＶＥＧＦ表达在不同的Ｂｏｒｒｍａｎｎ分型及ＴＮＭ分期中各不相同，并随着疾病的分期而增加（Ｐ＜０．０１）。提示：胃癌ＭＶＤ值和ＶＥＧＦ的表达密切相关，并与胃癌的病理及临床分期相联系，两者均可作为反映胃癌生物学行为的有用指标。The relationship between the microvessel density (MVD), the expression of vascular endothelial cell growth factor (VEGF) and the pathological types and clinical stages in gastric cancers was studied. The MVD and the expression of VEGF in the samples of gastric cancers were assayed by using immunohistochemical methods of SABC with human factor Ⅷ related antibody, the VEGF monocolonal antibody. The mean value of MVD and the positive rate of VEGF expression in gastric cancer were 31.4 ±14.3 and 53.3% (24/45) respec- tively, and the value of MVD in those with positive VEGF expression was markedly higher than that in those with negative VEGF expression (P<0.05). The value of MVD and the positive rate of VEGF expression were varied in different Borrmann types and TNM stages and were raised with the types and stages of gastric cancer improved (P<0.01 ). It was suggested that the MVD value in gastric carcinoma was closely related with the expression of VEGF and associated with the pathological types and clinical stages. Both of them could be used as the useful indexes reflecting the biological behavior of gastric carcinoma.

关 键 词：肿瘤微血管密度 血管内皮生长因子 胃癌 病理 

分 类 号：R735.202[医药卫生—肿瘤]