MMP-2、MMP-9酶活性与人肝细胞癌生物学行为的研究  

Study on Relationship between Activity of MMP-2(72KD),MMP-9(92KD) and Invasion Metastasis of Human Hepatocellular Carcinoma

作　　者：赵跃武[1] 孔令非[1] 唐华[1] 银平章[1] 

机构地区：[1]河南省人民医院病理科,郑州450003

出　　处：《肿瘤防治研究》2000年第5期370-372,共3页Cancer Research on Prevention and Treatment

摘　　要：目的　探讨MMP - 2 ( 72KD)、MMP - 9(KD)的活性与肝细胞癌 (HCC)浸润、转移的关系。方法　利用酶谱和计算机软件检测并分析了 32例肝细胞癌组织中MMP - 2、MMP - 9活性。结果　肝细胞癌 72KD的水平 ( 2 8 89± 7 2 8)高于癌旁组 ( 19 41± 5 52 ) ,P <0 0 5;肝癌组 62KD +72KD( 55 46± 11 64)与癌旁组 ( 2 8 0 6± 7 72 )比较时 ,P <0 0 5。同时发现 62KD恶性组 ( 2 6 57±9 3)明显高于癌旁组 ( 8 65± 1 98) ,P <0 0 5;转移组 62KD( 34 72 +13 52 )明显高于非转移组 ( 18±4 1) 62KD 62KD +72KD ;恶性组 ( 0 47)明显高于癌旁组 ( 0 31) ,P <0 0 1;转移组 ( 0 53)明显高于非转移组 ( 0 41) ,P <0 0 1。另外 ,肝细胞癌中 92KD的水平 ( 2 7 95± 11 93)明显高于癌旁组 ( 7 46±2 53) ,P均 <0 0 1;转移组 ( 35 59± 13 67)高于非转移组 ( 2 0 31± 10 18)P <0 0 1。结论　结果提示MMP - 2、MMP - 9的水平及活性与肝细胞癌的浸润和转移有关。To investigate lelationship between activity of MMP-2(72KD),MMP-9(92KD) and invasion,metastasis of human hepatocellular carcinomar(HCC).Methods Useing Zymography and computer software assisted analysis,we have measureed the activitive levels of MMP-2(72KD),MMP-9(92KD)in tissues from HCC.Results Mean activitive levels of 72KD(28 89±7 82)in HCC were higher than those in benign disease, P <0 05.There was difference( P <0 05). in 62KD+72KD of the tumorous tissues and the non-tumorous tissues.62KD(26 57±9 30)was markly higher in HCC ( P <0 01).We also fund 62KD/62KD+72KD were apparently higher in HCC(0 47) and metastases (0 41), P <0 01 ,respectively.Meanwhile,levels of 92KD were higher in HCC tiessues (27 95±11 93)than in nontumorous tissues(7 46±2 53), P <0 01.levels of 92KD were higher in metastases than in non-matastasis carcinomas, P <0 01,Conclusion These results demonstrate that levels of MMP-2,MMP-9 can be candicates for novel markeres for HCC.The levels appear to reflect their potential and angoing activity of invasion and metastasis of HCC.

关 键 词：肝细胞癌 MMP-2 MMP-9 酶谱 肿瘤转移 肿瘤浸润 

分 类 号：R735.7[医药卫生—肿瘤]