人乳腺癌细胞株MCF-7、MCF-7/ADM细胞迁移能力的对比研究  

Comparative analysis of cell migration of human breast cancer cell line MCF-7 and MCF-7/ADM

作　　者：陈平[1] 李克强[1] 毛联钢[1] 乐东海[1] 冯伟云[1] 

机构地区：[1]宁波市第二医院普外科,315000

出　　处：《浙江医学》2013年第23期2050-2053,共4页Zhejiang Medical Journal

基　　金：宁波市自然科学基金项目(2009A610179)

摘　　要：目的探讨乳腺癌细胞化疗继发耐药后细胞迁移能力的变化,并筛选出相关基因。方法以MCF-7细胞株为亲本细胞。采用阿霉素(ADM)低浓度持续加量诱导法建立对ADM耐药的人乳腺癌细胞株MCF-7/ADM。应用ATP-TCA药物敏感检测法和xCELLligence/RT-CES实时细胞电子分析系统检测人乳腺癌细胞株MCF-7、MCF-7/ADM对多种化疗药物的耐药情况及细胞增殖能力。应用Transwell实验和xCELLligence/RT-CIM实时细胞电子分析系统检测人乳腺癌细胞株MCF-7、MCF-7/ADM的细胞迁移能力。应用比较基因组芯片筛选出人乳腺癌细胞株MCF-7、MCF-7/ADM DNA拷贝数4倍差异的基因。结果撤药培养150d后,MCF-7/ADM细胞较亲本细胞MCF-7的ADM耐药指数为72.9,对其他化疗药物无明显的交叉耐药性。MCF-7/ADM细胞迁移能力较MCF-7明显降低(P<0.05)。MCF-7/ADM有12种基因DNA拷贝数是MCF-7的4倍,MCF-7有6种基因DNA拷贝数是MCF-7/ADM的4倍。结论乳腺癌细胞化疗继发耐药伴随细胞迁移能力明显降低,其相关基因的DNA拷贝数亦有明显差异。Objective To investigate the change of the cell migration ability of breast cancer cells with acquired resistance to chemotherapy, and screen the related genes. Methods MCF-7 cells were used as the parental cells, and human breast can- cer cell line MCF-7/ADM were established with continuous low concentration of ADM. The chemotherapy resistance of MCF-7 and MCF-7/ADM cell was detected by ATP-TCA drug sensitive test and xCELLligence/RT-CES real-time cerl electronic analysis system. The cell migration of MCF-7 and MCF-7/ADM cell was detected by Transwell test and xCELLligence/RT-CIM real-time celt electronic analysis system. 4 times the difference of DNA copies of Genes in MCF-7 and MCF-7/ADM cell were screeninged by comparative genomic chip. Results The drug resistant index of MCF-7/ADM cell kept on 72.9 times over MCF-7 cell after cultured in the medium （without ADM） for 150 days. MCF-7/ADM had no obvious cross-resistance to other chemotherapy drugs. The cell migration of MCF-7/ADM reduced significantly than that of MCF-7 （P〈0.05）. The DNA copies of 12 genes of MCF-7/ADM cell are 4 times more than that of MCF-7, while the DNA copies of 6 genes of MCF-7 cell are 4 times more than that of MCF-7/ADM Conclusion The migration ability of breast cancer cells was significantly reduced with acquired chemotherapy resistance, and the DNA copies of some genes are also obvious different.

关 键 词：乳腺癌 阿霉素 化疗耐药 细胞迁移 

分 类 号：R737.9[医药卫生—肿瘤]