喉癌下咽癌TGFβ_1、p16表达的意义  

EXPRESSIONS OF TGF β_1 AND p16 IN LARYNGEAL AND HYPOPHARYNGEAL CARCINOMA

作　　者：郑美桦[1] 栾信庸[1] 田晓东[1] 

机构地区：[1]山东医科大学附属医院耳鼻喉科

出　　处：《山东医科大学学报》2000年第4期381-383,387,共4页Acta Academiae Medicinae Shandong

基　　金：山东省卫生厅科研基金

摘　　要：目的 :通过对喉癌、下咽癌组织中转化生长因子 β1(TGFβ1)及 p16蛋白的定位、半定量研究 ,探讨其在喉癌、下咽癌组织中的表达与意义。方法 :采用SABC免疫组化法研究TGFβ1与 p16蛋白的表达 ,并对表达结果进行统计分析。结果 :TGFβ1位于胞浆 /胞膜 ,p16蛋白位于胞浆 /胞核。TGFβ1强表达率为61% ,p16蛋白强表达率为 66%。高分化与中、低分化肿瘤的TGFβ1表达均有统计学差异 (P <0 .0 5) ;高分化与低分化肿瘤的 p16蛋白表达具统计学差异 (P <0 .0 5)。TGFβ1和 p16蛋白之间存在正相关系 (P <0 .0 5)。TGFβ1和 p16蛋白的表达与患者年龄、性别、肿瘤生长部位、病程、临床分期无明显相关。 结论 :TGFβ1与 p16蛋白在喉癌、下咽癌中存在缺失表达 ,在高分化肿瘤中有更高的表达 ,在细胞周期中具有共同的作用。Objective:To study the expressions of TGFβ 1 and p16 in laryngeal and hypopharyngeal carcinoma and reveal the relationship between the expressing levels and the grades,age,sex,disease periods,cancer position,clinical stages.Methods:Surgical specimens were obtained from 43 patients with laryngeal squamous cell carcinoma and 18 patients with hypopharyngeal squamous cell carcinoma.By immunohistochemistry on paraffin embeded tissue sections,the presence of p16 and TGFβ 1 with monoclonal antibodies was investigated.Results:The p16 positive stain was mainly located in cytoplasma.The positive rate was higher in well differentiated carcinomas than in poorly differentiated ones(P<0.05).The expression level of TGFβ 1 at intracellular was significantly higher in well differentiated carcinomas than in poorly and moderately differentiated ones (P<0.05).The expressions of p16 and TGFβ 1 were positively related (P<0.05).Conclusion:The expressions of p16,TGFβ 1 are associated with malignant phenotype in laryngeal and hypopharyngeal carcinomas.TGFβ 1 and p16 play important roles in cell cycle regulating.

关 键 词：喉癌 下咽癌 P16 原癌基因蛋白 转化生长因子Β 

分 类 号：R739.65[医药卫生—肿瘤] R739.63[医药卫生—临床医学]