单纯疱疹病毒胸苷激酶基因转染联合药物对卵巢上皮癌细胞杀伤作用的研究  

THE KILLING EFFECT OF RETROVIRUS-MEDIATED HSV_(1)-tk GENE TRANSDUCTION FOLLOWED BY ADMINISTRATION OF DRUG ON OVARIAN CANCER CELLS

作　　者：姜洁[1] 孔北华[1] 江森[1] 刘春生[2] 

机构地区：[1]山东医科大学附属医院妇产科 [2]山东医科大学附属医院肿瘤防治研究中心

出　　处：《山东医科大学学报》2000年第4期384-387,共4页Acta Academiae Medicinae Shandong

基　　金：山东省优秀中青年科学家科研奖励基金资助项目

摘　　要：目的 :探讨逆转录病毒载体介导的单纯疱疹病毒胸苷激酶 (HSV1 tk)基因转染 ,联合抗病毒药物羟甲基无环鸟苷 ( ganciclovir ,GCV)和无环鸟苷 (acyclovir ,ACV )对人卵巢上皮癌 (OEC)细胞系TYK的杀伤作用。方法 :用lipofectionreagent介导法将PLNTK5质粒转移入包装细胞PA317中 ,用滴度最高的PA317病毒上清液转染TYK细胞 ,运用MTT法及光镜、电镜检测GCV、ACV对目的细胞的体外杀伤效应。结果 :将PLNTK 5质粒转入PA317细胞后 ,得到了稳定的产病毒细胞株。TYK细胞可被病毒上清液转染 ,转导HSV1 tk基因可显著增强ACV、GCV对TYK细胞的杀伤作用。结论 :逆转录病毒可介导HSV1 tk基因转染TYK细胞并获稳定表达 ,ACV、GCV对携有HSV1 tk基因的卵巢癌细胞有明显的体外杀伤作用。Objective:To evaluate the treatment effect of retrovirus mediated herpes simplex virus thymidine kinase(HSV 1 tk) gene transference followed by administration of ganciclovir(GCV) and acyclovir(ACV) on ovarian epithelial cancer cells.Methods:PLNTK5 plasmid was transferred into an amphoteric packing cell line PA317 using lipofectin reagent method.Ovarian epithelial cancer cell line TYK was infected by the PA317 budded virus whose titer was the highest.The cytotoxicity efficacy of HSV 1 tk/GCV,ACV system on TYK cell line was evaluated using microcucture tetrajolium test(MTT) method and electron microscope.Results:Stable virus producing cell line was established after PLNTK5 plasmid was transferred into PA317.The TYK cell was infected by the PA317 budded virus.In vitro study showed that the HSV 1 tk gene transfection increased the efficacy of GCV and ACV on TYK cells.Conclusion:The HSV 1 tk gene can be transfected into human ovarian cancer cell line under the mediation of retrovirus.GCV and ACV have significant inhibiting efficacy in vitro on TYK/tk cells.

关 键 词：卵巢肿瘤 细胞系 胸苷激酶 单纯疱疹病毒 逆转录病毒科 

分 类 号：R737.3[医药卫生—肿瘤] R730.5[医药卫生—临床医学]