BOP诱导叙利亚仓鼠胰腺癌动物模型建立和相关生物学特性的研究  

The biological characteristics related study of BOP-Induced syrian golden hamsters pancreatic cancer

作　　者：卫积书[1] 张烨[1] 任华建[1] 吕楠[1] 蔡宝宝 吴鹏飞[1] 彭云鹏[1] 李强[1] 苗毅[1] 

机构地区：[1]南京医科大学第一附属医院普外科,江苏南京210029

出　　处：《南京医科大学学报（自然科学版）》2013年第12期1669-1673,共5页Journal of Nanjing Medical University(Natural Sciences)

基　　金：国家自然科学基金(81272239);卫生公益性行业科研专项经费(201202007)资助

摘　　要：目的:探讨N-亚硝酸2-双丙基胺[bis-(2-oxopropyl)-N-nitro samine,BOP]诱导建立叙利亚仓鼠胰腺癌动物模型的可行性和建模过程特点,初步分析模型胰腺癌的相关生物学特点,为胰腺癌动物模型研究提供一个可控性强、动态、全面的基础。方法:73只叙利亚仓鼠随机分为实验组和对照组,实验组予以生理盐水稀释成20 mg/ml的BOP按照20 mg/kg体重腹壁皮下注射每周1次,连续4周;相同条件注射生理盐水为对照组。连续监测各组仓鼠体重,注射结束后4周开始每2周依次收集两组叙利亚仓鼠组织标本,观测其形态特点。结果:注射后20周实验组开始出现胰腺肿瘤,注射后28周开始成瘤明显增加,病理观测为低分化腺癌,复种实验成功,部分实验结果与既往报道不一致。结论:BOP可以成功构建叙利亚仓鼠胰腺低分化腺癌动物模型,在注射后第28周后收集标本时间最佳,部分实验结果值得进一步深入研究。Objective： To investigate the feasibility of BOP [bis-（2-oxopropyl）-N-nitro samine]-induced Syrian hamsters pancreatic adenocarcinoma model,and explore the character of the modeling process. Preliminary analysis of related biology characteristics provides a controllable,dynamic and comprehensive foundation for BOP induced pancreatic animal model. Methods：Seventy-three syrian golden hamsters were treated with carcinogen,N-nitrosobis（2-oxopropyl） amine weekly for 4 weeks（20 mg/kg,subcutaeous injection）,and sacrificed at 2-week intervals after 4-week from the last injection. Results：The experimental group pancreatic tumors began to appear after 20 weeks after first injection,different from previous reports,and tumorigenicity was significant higher in 28 weeks after injection,Primary tumor specimen implantation verified tumorigenicity,parts of experimental results were inconsistent with previous reports. Conclusion：BOP can successfully induce Syrian hamsters poorly differentiated pancreatic adenocarcinoma,and the 28th week after first time injection was the optimally time for sample collection. Some experimental results are worthy of further in- depth study.

关 键 词：N-亚硝酸2-双丙基胺 叙利亚仓鼠 胰腺癌 动物模型 

分 类 号：R735.9[医药卫生—肿瘤]