利奈唑胺体外诱导金黄色葡萄球菌耐药的实验研究  

The drug-resistance of Staphylococcus aureus induced by linezolid in vitro

作　　者：李艳芬[1] 王俊[1] 王淑香[1] 郭文学[1] 祁伟[1] 

机构地区：[1]天津医科大学第二医院感染性疾病研究所,天津市300211

出　　处：《中华实验和临床感染病杂志（电子版）》2013年第5期60-64,共5页Chinese Journal of Experimental and Clinical Infectious Diseases(Electronic Edition)

摘　　要：目的体外诱导金黄色葡萄球菌对利奈唑胺耐药,研究分析其耐药后菌株变化。方法用利奈唑胺浓度2倍递增的方法对9株临床分离金黄色葡萄球菌(5株MSSA、4株MRSA)和质控菌株ATCC25923进行体外诱导;PCR扩增诱导前后金黄色葡萄球菌23S rRNA的V区基因并测序、Blast比对,分析突变与耐药性的关系;比较诱导前后金黄色葡萄球菌的适应性以及对抗菌药物的耐药性变化。结果 10株实验菌株中有9株诱导出稳定的利奈唑胺耐药性,其中5株耐药菌发生了突变;利奈唑胺耐药后金黄色葡萄球菌生长速率减慢,对其他抗菌药物耐药性也发生改变。结论经利奈唑胺诱导后金黄色葡萄球菌可以获得稳定耐药性,但同时也产生了适应性代价,其耐药机制与23S rRNA的V区基因突变相关,并且可能有其他耐药机制的参与。Objectives To investigate the in vitro linezolid-induced resistance in Staphylococcus aureus and its change. Methods Linezolid in vitro induction was conducted with 9 S. aureus isolates （5 MSSA, 4 MRSA） and 1 reference strain. Amplification and sequencing of domain V 23S rRNA gene. To compare the fitness of S. aureus, and the susceptibility against other antibiotics. Results Resistance to linezolid was successfully in 9 of 10 strains. Point mutation was identified in 23S rRNA gene in 5 linezolid-resistant strains. The linezolid-resistant S. aureus grew much slower than the susceptible parent, and definite changes in the susceptibility were demonstrated. Conclusions S. aureus can be induced resistance in vitro by linezolid, and this resistance may be associated with the point mutation of 23S rRNA gene. The fitness cost observed in the linezolid-resistant strains.

关 键 词：金黄色葡萄球菌 利奈唑胺 耐药性 基因突变 适应性代价 

分 类 号：R965[医药卫生—药理学]