慢病毒介导CD-TK融合基因转染神经干细胞后杀伤C6胶质瘤的研究  

Effect of Neural Stem Cells Modified by CD-TK Gene Mediated by Lentivirus on Glioma Cells

作　　者：闫超[1] 刘昊[1] 彭海声 牛健[1] 邢春阳[1] 李殿俊[3] 陶海泉[1] 杨海城[1] 

机构地区：[1]哈尔滨医科大学附属第二医院,黑龙江哈尔滨150086 [2]哈尔滨医科大学大庆校区药学院,黑龙江大庆163019 [3]哈尔滨医科大学免疫学教研室,黑龙江哈尔滨150086

出　　处：《中国肿瘤》2014年第1期63-67,共5页China Cancer

摘　　要：[目的]观察慢病毒介导的CD-TK融合基因转染神经干细胞(NSCs)对C6胶质瘤生长的抑制作用以及疗效。[方法]利用慢病毒介导的大肠杆菌胞嘧啶脱氨酶(CD)和单纯疱疹病毒胸苷激酶(HSV-TK)融合基因转染NSCs,植入C6胶质瘤模型。用流式细胞仪技术检测C6胶质瘤生长凋亡情况。[结果]成功构建CD-TK基因转染NSCs模型。实验组坏死细胞(17.80%)、早期凋亡细胞及晚期凋亡细胞(33.30%)较对照组增加。CDglyTK-NSC+GCV/5-Fc治疗组肿瘤体积为9.36±2.35mm3,小于其他处理组及对照组的肿瘤体积(P<0.05)。[结论]转染NSCs的CD-TK基因对C6胶质瘤细胞有抑制作用,抗肿瘤效果明显,优于单一的自杀基因系统。[ Purpose] To investigate the inhibition effect and efficacy of neural stem cells modified by CD-TK gene mediated by lentivirus in the treatment of glioma. [Methods] CD-TK gene was in- troduced into NSCs by lentivirus-mediated transfection and implanted into the model of C6 glioma. FCM was used to observe the apoptosis of glioma in vivo. [Results] NSCs/CD-TK model was successfully eonstrueted. Necrotic cells,apoptotic cells in treatment group were significantly higher than those in other groups（P〈0.05）.Tumor volume in CDglyTK-NSC＋GCV/5-Fc group （9.36±2.35mm3）was smaller than that in other groups（P〈0.05）. [Conclusionl NSCs/CD-TK signifi cantly inhibited the growth of C6 cells with a better anti-tumor effieaey than CD-TK suicide gene.

关 键 词：CD-TK融合基因 神经干细胞 胶质瘤 基因治疗 

分 类 号：R739.41[医药卫生—肿瘤]