斑蝥素与斑蝥多肽抗肿瘤活性比较  

Comparative Study of Antitumor Activity of Cantharidin and Cantharis Peptides

作　　者：廖秀英[1] 陆颂规 封家福[1] 

机构地区：[1]乐山职业技术学院,四川乐山614000 [2]广州白云山敬修堂药业股份有限公司,广东广州510120

出　　处：《中药材》2013年第10期1566-1569,共4页Journal of Chinese Medicinal Materials

基　　金：广东省科技计划项目(2011KT480)

摘　　要：目的:从中药斑蝥中制备斑蝥多肽及斑蝥素,并对其抗肿瘤活性进行比较。方法:采用仿生酶解法制备斑蝥多肽,碱性水液超声提取斑蝥素;采用Prestoblue法检验两组物质对人肝癌BEL-7402细胞增殖的抑制作用;检验两组物质对S180荷瘤小鼠的肿瘤、胸腺、脾脏生长的影响。结果:斑蝥多肽和斑蝥素体外能抑制BEL-7402细胞的增殖(P<0.05),斑蝥多肽的抑制率较斑蝥素低12.54%;体内能抑制S180肉瘤的生长(P<0.05),斑蝥素的抑制率较斑蝥多肽高5.93%,且斑蝥多肽对胸腺和脾脏的生长无抑制。结论:斑蝥多肽和斑蝥素均有抗肿瘤活性,但斑蝥多肽无免疫抑制。Objective： To prepare cantharidin and cantharidin peptides from Mylabris and compare their antitumor activity. Methods ： Cantharis peptides was prepared by bionic enzymolysis approach and cantharidin was prepared by alkaline water supersonic extraction. The inhibitory effects of both compounds on BEL-7402 cells proliferation of human liver cancer were tested by Prestoblue method. The influence of both compounds on the grown of tumor, thymus, spleen of S180 tumor-bearing mice were detected. Results：Cantharis peptides and cantharidin could inhibit the proliferation of BEL-7402 cells （ P 〈 0. 05 ） in vivo, and the inhibitory effect of cantharis peptide was 12. 54% lower than that of cantharidin. At the same time, they could inhibit the grown of Siso sarcoma （P 〈 0. 05 ）, and the inhibitory effect of cantharidin was higher than that of cantharis peptides （5.93%）. Furthermore, cantharis peptides could＇t inhibit the grown of thymus and spleen. Conclusion：Both cantharis peptides and cantharidin have antineoplastic activity, but cantharidin peptides have no imnmnosuppression.

关 键 词：斑蝥 多肽 斑蝥素 抗肿瘤 Prestoblue法 

分 类 号：R285.5[医药卫生—中药学]