改进计时电流法对电化学PCR仪试剂温度的实时监测  

Real time monitoring of reagent temperature on electrochemical PCR instrument by improved chronoamperometry

作　　者：柯栋忠[1,2] 刘绍丽[2,3] 陈旭海[3,4] 陈敬华[5] 张蓬勃[2] 杜民[2] 林新华[5] 

机构地区：[1]福州大学物理与信息工程学院,福建福州350116 [2]福建省医疗器械和医药技术重点实验室,福建福州350002 [3]湖北文理学院理工学院,湖北襄阳441000 [4]福建省电力勘测设计院,福建福州350003 [5]福建医科大学药学院,福建福州350108

出　　处：《福州大学学报（自然科学版）》2013年第6期1016-1022,共7页Journal of Fuzhou University(Natural Science Edition)

基　　金：国家高技术研究发展计划资助项目(2008AA02Z433);国家自然科学基金资助项目(21105012);福建省自然科学基金资助项目(2011J01028);福建省科技厅重点资助项目(2010I0017);福建省高校杰出青年科研人才培育计划资助项目(JA11105)

摘　　要：通过改进计时电流法的精确模型数值解仿真,准确分析得到响应电流峰值信号与被测物浓度及电路系统装置相关参数的关系.研究发现,采用改进电路装置检测试剂电化学反应的电流峰值信号,除与试剂浓度有关外,还受试剂温度的影响.而理论模型中能斯特方程和扩散定律包含温度参量,因此数值解还可作为确定电流与温度变化关系的依据.采用改进CA法快速检测试剂温度的变化,有效提高PCR仪测温装置的实时性,并且降低测温的复杂性.Based on a numerical model of improved chronoamperometry, the relationship between the working currents and detection reagent concentration was analyzed quantitatively, and the dependence of peak currents on parameters for the improved system was also represented. We found that the cur- rents were not only changed with the reagents concentration, but also were influenced by the tempera- ture during the whole experiments. Both Nernst equation and Flick＇ s law contain temperature, so the numerical model could also be used to find out the law between currents and temperature. Experimen- tal results show that the improved CA model could indicate real - time temperature change of detection reagent. This research efficiently increases the real - time performance of the PCR instrument and de- creases its complexity.

关 键 词：改进计时电流法 聚合酶链式反应 试剂温度 能斯特方程 扩散系数 

分 类 号：R318[医药卫生—生物医学工程]