Twist、E-cadherin在原发性乳腺癌组织中的表达及相关性研究  

Expression and correlation of Twist and E-cadherin in primary breast cancer

作　　者：秦优优[1] 杨学伟[1] 李妍[1] 闫朝岐[1] 张东伟[1] 崔武[1] 

机构地区：[1]哈尔滨医科大学附属第二医院普外科,150086

出　　处：《临床外科杂志》2013年第11期853-856,共4页Journal of Clinical Surgery

基　　金：黑龙江省自然科学基金（D201145）

摘　　要：目的 观察Twist蛋白和E-cadherin蛋白在乳腺癌组织中的表达情况,探讨二者在乳腺癌浸润转移过程中的作用及关系.方法 应用免疫组织化学方法（SP法）检测60例乳腺浸润性导管癌组织和30例乳腺癌旁正常组织中Twist蛋白和E-cadherin蛋白的表达情况,分析二者与乳腺癌临床病理因素的关系.结果 Twist蛋白与E-cadherin蛋白在乳腺癌组织中的阳性表达率分别为63.3％、71.6％,在癌旁正常组织中的阳性表达率分别为16.7％、90.0％,差异有统计学意义（P＜0.05）.Twist蛋白及E-cadherin蛋白的表达与患者年龄、肿块大小均无明显相关性（P＞0.05）,与临床分期、组织学分级和淋巴结转移具有显著相关性（P＜0.05）.Spearman相关性检验示二者之间呈负相关（P＜0.05）.结论 在原发性乳腺癌组织中,Twist蛋白呈高表达,E-cadherin蛋白呈低表达,均与乳腺癌临床分期、组织学分级和腋窝淋巴结转移情况有相关性,且二者表达呈显著负相关.提示Twist蛋白可能通过调控E-cadherin的表达,进而调控肿瘤细胞的EMT过程,促进乳腺癌的发生及转移.Objective To observe the expression of Twist and E-cadherin in breast cancer and discuss the correlation and significance in invasion and metastasis. Methods The expression of Twist and E-cadherin was observed by SP immunohistochemical technique for 60 cases of primary breast carcinomas and 30 cases of corresponding non-tumor normal tissues. The relationship between the proteins and clinico- pathological characteristics was analysed. Results The positive rates of Twist and E-cadherin expression were 63.3% and 71.6% in breast cancer,and 16.7% and 90% in corresponding non-tumor normal tis- sues （P 〈 0.05 ）. The expression of Twist and E-cadherin had no correlation with patients＇age or tumor size （P 〉 0.05 ）, but it had correlations with clinical stage, histological grade and lymph node metastasis （P 〈 0.05 ）. The Spearman interclass correlation analysis showed that the expression of Twist is negative correla- ted with that of E-cadherin （P 〈 O. 05 ）. Conclusion The expression of Twist is increased and E-cadherin is decreased in breast cancer, and they are significantly correlated with clinical stage, histological grade and axillary lymph node metastasis in breast cancer. The expression of Twist is significantly negative correlated with that of E-cadherin, which indicates that Twist may regulate the expression of E-adherin,induce epithe- lial-mesenchymal transition（EMT） in tumor cells,and finally result in the invasion and metastasis.

关 键 词：乳腺癌 TWIST E-CADHERIN 浸润转移 

分 类 号：R737.9[医药卫生—肿瘤]