低强度微波辐射致兔晶状体上皮细胞超微结构的早期改变  

Ultrastructural change of rabbit lens epithelial cells induced by low power level microwave radiation

作　　者：叶娟[1] 姚克[1] 吴仁毅[1] 姚耿东[2] 

机构地区：[1]浙江大学医学院附属第二医院眼科中心,杭州310009 [2]浙江大学医学院生物电磁学研究室

出　　处：《中华眼科杂志》2001年第1期56-58,T002,共4页Chinese Journal of Ophthalmology

摘　　要：目的　探讨低强度微波辐射致兔晶状体上皮细胞超微结构的改变及其发生机制。方法采用 10mW/cm2 低强度微波照射 8只兔眼 30h后 ,在透射电镜下观察兔晶状体上皮细胞超微结构的改变 ,并与自身对照眼比较。结果　受照射眼晶状体上皮细胞发生胞体缩小 ,核膜内陷 ,线粒体数量减少、呈空泡状 ,内质网扩张等细胞凋亡的超微结构改变 ,自身对照眼细胞结构无明显改变。结论低强度 (10mW/cm2 )微波辐射可导致兔晶状体上皮细胞超微结构发生变化 ,其可能是晶状体上皮细胞不可逆损伤的早期表现 ,发生机制尚需深入研究。Objective To study the ultrastructural change of rabbit lens epithelial cells with transmission electron microscope and to observe whether low power level microwave can induce the injury of rabbit lens Methods Eight rabbit eyes were exposed to 10 mW/cm 2 low power level microwave radiation for 30 hours Transmission electron microscope was used to observe the ultrastructural changes of the lens epithelial cells The other eye was served as self control Results The ultrastructure of exposed lens epithelial cells changed apparently The characteristic apoptosis cell morphological changes, such as small cell size, the decreased number of mitochondria, balloon shaped mitochondria, etc can be seen No obvious changes can be found in the protected self control eyes Conclusions The low power level microwave (10 mW/cm 2) can induce the ultrastructural changes of rabbit lens epithelial cells, this may be the appearance of early irreversible microwave radiation injury In regard to the pathogenic mechanism, it is necessary to perform further investigations

关 键 词：晶体上皮细胞 低强度微波辐射 白内障 超微结构 兔 

分 类 号：R776.1[医药卫生—眼科]