人脑胶质瘤GDNF基因启动子Ⅰ区H_3组蛋白乙酰化修饰情况  

Situation of GDNF Promoter I Region in the Human Brain Glioma in H3K9Ac Modification

作　　者：刘泽昊[1] 虞正权[1] 高殿帅[2] 贾阳[1] 陈传新[1] 

机构地区：[1]苏州大学附属第一医院神经外科,江苏苏州215006 [2]徐州医学院神经生物实验室,江苏徐州221002

出　　处：《现代生物医学进展》2013年第35期6818-6821,共4页Progress in Modern Biomedicine

基　　金：江苏省高校自然科学基金项目(11KJB180012);青年科学基金项目(81101899)

摘　　要：目的:明确GDNF启动子Ⅰ区在人脑胶质瘤中H3赖氨酸残基9位乙酰化(H3K9Ac)情况,探讨其对于GDNF在胶质瘤中表达的影响。方法:RT-PCR检测各组中GDNF mRNA的表达;建立基于Real-time PCR分析的染色质免疫共沉淀(ChIP)方法,检测12例胶质瘤与6例正常脑组织中GDNF基因启动子Ⅰ区H3组蛋白乙酰化情况。结果:Real-time PCR验证人脑胶质瘤GDNF mRNA的表达,转录水平随级别的增高而增高,且低级别组、高级别组与正常组之间存在显著的统计学差异(P<0.05)。启动子Ⅰ区的H3组蛋白乙酰化水平,正常组与低级别组和高级别组之间比较均有显著性差异(P<0.05),且低级别与高级别之间也有显著性差异。结论:在人脑胶质瘤组织中,GDNF启动子Ⅰ区发生了H3组蛋白高乙酰化修饰,这种修饰很可能会影响GDNF基因的表达。Objective： To observe the histoneacetylation of GDNF promoter 1 region in human glioma, in order to explore the effect of methylation on the expression of GDNF in glioma. Methods： RT - PCR detection in each group GDNF mRNA expression; Based on the analysis of the Real - time PCR chromatin immune coprecipitation （CHIP） method, testing 12 cases of gliomas and 6 cases of normal brain tissue GDNF gene promoter region H3 histone acetylation situation. Results： Real - time PCR test the brain glioma GDNF mRNA expression shows that transcription level with the level of higher and higher. The low level group, high level group and normal group there was significant statistical difference （P〈0.05）. The promoter I H3 histone acetylation level, there was significant difference （P〈0.05） among the normal group, low level group and high level group, and there was also significant difference between low level and high level, Conclusion： Acetylation occuerred in GDNF promoter I region might influence the expression of GDNF in glioma

关 键 词：胶质瘤 胶质细胞源性神经营养因子 启动子 乙酰化 

分 类 号：R739.41[医药卫生—肿瘤]