红景天苷对LPS诱导的HSC-T6细胞增殖作用及机制研究  

Study on Effects and Mechanism of Salidroside on Proliferation of Lipopolysaccharide-induced Hepatic Stellate Cells

作　　者：陈磊[1] 王乐[2] 张天翼[1] 曹农[1] 陈建宗[3] 

机构地区：[1]兰州大学第一附属医院普通外科,甘肃兰州730000 [2]第四军医大学唐都医院中医科,陕西西安710038 [3]第四军医大学西京医院中医药研究中心,陕西西安710032

出　　处：《现代生物医学进展》2013年第35期6826-6828,共3页Progress in Modern Biomedicine

基　　金：国家自然科学基金项目(30772743)

摘　　要：目的:观察红景天苷(Salidroside,Sal)对大鼠肝星状细胞(hepatic stellate cell,HSC)-T6增殖的影响,并探讨一氧化氮(NO)在此过程中的作用。方法:选择脂多糖(LPS)活化HSC-T6,采用不同浓度的Sal,作用于经LPS活化的大鼠HSC-T6,24小时后进行如下实验:噻唑兰(MTT)比色法检测HSC-T6增殖;NO荧光探针(DAF-FM DA)检测细胞内NO浓度;Western-blot检测INOS蛋白水平。结果:在LPS作用于HSC-T6细胞24小时后,与对照组相比,细胞增殖增加(P<0.01),在Sal作用下,HSC-T6细胞增殖与LPS组相比受到抑制(P<0.01),并呈浓度依赖性;在Sal处理HSC-T6细胞24小时后,细胞内NO水平有所上升,并呈浓度依赖性增加(P<0.01);Sal预处理后的HSC-T6细胞,iNOS蛋白表达有所升高(P<0.01)。结论:Sal对HSC-T6细胞的增殖具有浓度依赖性的抑制作用,NO可能是抑制增殖的因素之一。Objective： To investigate the effect of Salidroside on proliferation of hepatic stellate Cells （HSC） and estimate the function of the NO during the procedure. Methods： Choosing the lipopolysaccharide （LPS）used to activate HSC-T6. Rat HSC-T6 activated by LPS were incubated with various concentration of Sal. Then conduct the following experiment after twenty-four hours： MTT colorimetric assay was used to detect the Proliferation of HSC-T6; The intracellular NO was detected using DAF-FM DA. Western blot were used to investigate the expression of iNOS protein. Results： Compared with the control group, the ratio of cell proliferation increased （P〈0.01） after LPS-induced HSC-T6 for 24h. Compared with the LPS group, the ratio of cell proliferation decreased（P〈0.01） after Sal treatment with LPS-induced HSC-T6 for 24h, and in a dose-dependent manner. In group treated with Sal, the NO concentration and expression level ofiNOS in HSC-T6 increased （P〈0.01）, and in a dose-dependent mariner（P〈0.01）. Conclusion： Sal could inhibit the proliferation of HSC-T6, which could be mediated by NO partially at least.

关 键 词：红景天苷 HSC-T6 增殖 一氧化氮 

分 类 号：Q95-3[生物学—动物学] R575.2[医药卫生—消化系统]