重组鲑鱼降钙素结肠黏附缓释微球的制备  

Preparation of Colon Adhesive and Sustainably Releasing Microsphere of Recombinant Salmon Calcitonin

作　　者：宋益民[1] 范鸣浩[1] 杨青[1] 张学成[2] 

机构地区：[1]青岛科技大学制药工程系,山东青岛266042 [2]中国海洋大学生命学院,山东青岛266003

出　　处：《中国海洋大学学报（自然科学版）》2014年第1期57-61,共5页Periodical of Ocean University of China

基　　金：国家自然科学基金项目(30471317)资助

摘　　要：为考察重组(酵母)鲑鱼降钙素结肠黏附缓释微球的制备工艺和最优处方,并对释药特性进行探讨。以阿拉伯胶、β-环糊精、可溶性淀粉、明胶为壁材,HPMC和卡波姆为生物黏附剂,运用正交试验确定喷雾干燥法制备微球的最佳工艺条件和处方。结果表明,微球的最佳制备工艺和处方为β-环糊精与阿拉伯胶以15∶1、HPMC与CP以4∶1配比为理想壁材,喷雾干燥进风温度160℃、进料速度10mL·min-1、雾化压力0.5MPa、壁材浓度15%、芯材比1∶3为最佳组合,所得微球能达到缓释12h的试验设计要求。本法工艺稳定、可行,所得鲑鱼降钙素结肠黏附缓释微球具有良好的缓释效果。In order to develop a preparation technique and optimize the formula of colon adhesive and sus- tainably releasing microsphere of salmon calcitonin synthesized in yeast （Saccharomyces cerevisiae） and characterize its in vitro releasing process, gum acacia, β-cyclodextrin, soluble starch powder and gelatin were tentatively used as encapsulation materials and HPMC and Carbomer as adhesive mediators. As was determined in orthogonal experiments, the most appropriate technique was spray drying with inlet air tem- perature set at 160 ℃, material feeding rate at 10 ml·min-1 and atomizing pressure at 0. 5 MPa. The op- timized encapsulation material was β-cyclodextrin and gum acacia （15·1） and adhensive mediator （core material） was HPMC and Carbomer （4：1）. Before spray drying, the encapsulation and core material were dissolved in water to a final concentration released salmon calcitonin for 12 h, meeting the ble and the formula optimized was applicable. of 15% and 5%, respectively. The prepared microsphere designed performance. The technique developed was feasi-ble and the formula optimized was applicable.

关 键 词：鲑鱼降钙素 转基因酵母 喷雾干燥 结肠黏附 缓释微球 体外释放 

分 类 号：R944.9[医药卫生—药剂学]