循环miRNA21在肝细胞癌早期诊断中的价值  

Circulating microRNA-21 as a novel biomarker for hepatocellular carcinoma

作　　者：秦志辉 朱喜丹[2] 黄远帅[2] 

机构地区：[1]泸县疾病预防控制中心,四川泸县646000 [2]泸州医学院附属医院检验科,四川泸州646000

出　　处：《四川医学》2013年第9期1423-1425,共3页Sichuan Medical Journal

摘　　要：目的探讨血浆中miRNA21作为肝细胞癌早期生物标志物的诊断价值。方法采用定量RT-PCR(quantitative RT-PCR)检测55例肝细胞癌患者、60例慢性肝炎患者、50例健康对照者血浆中miRNA21的表达水平。结果血浆miRNA21在肝细胞癌组中水平显著高于慢性乙肝组(P<0.01)和健康对照组(P<0.01)。通过绘制受试者工作曲线(receiver-operator characteristic curve,ROC curve)分析,miRNA21作为肝细胞癌和慢性乙肝的诊断标志物,其诊断价值分别为:灵敏度(65%),特异度(85%),受试者工作曲线下面积(AUC)为0.771,而miRNA21作为肝细胞癌和健康对照者的诊断标志物,其诊断价值分别为:灵敏度(89%),特异度(95%),AUC为0.956。结论 miRNA21是肝细胞癌潜在的早期诊断标志物。Objective The aim of this study was to evaluate the significance of plasma microRNA-21 level as a biochemi- cal marker for hepatocellular carcinoma. Methods Plasma microRNA-21 level was measured by RT-PCR. And it was compared in three groups： 55 patients with hepatocellular carcinoma, 60 patients with chronic hepatitis, and 50 healthy volunteers. Results Plasma microRNA-21 level in 55 patients with hepatoeellular carcinoma was significantly higher than in patients with chronic hepatitis and healthy volunteers （P 〈 0.01, P 〈 0.01, respectively）. Receiver-operator characteristic curve analysis of plasma mi- cro-RNA-21 yielded an AUC of 0.771 with 65% sensitivity and 85% specificity when differentiating hepatoeellular carcinoma from chronic hepatitis, and an AUC of 0. 956 with 89% sensitivity and 95% specificity when differentiating hepatocellular carcinoma from healthy volunteers. Conclusion Plasma microRNA-21 level might be a promising biochemical marker for hepatocellular car- cinoma.

关 键 词：肝细胞癌 微小RNA21 灵敏度 特异度 

分 类 号：R735.7[医药卫生—肿瘤]