体外产气法研究半胱胺对水牛瘤胃发酵参数和甲烷产量的影响  

Effects of Cysteamine on Ruminal Fermentation Parameters and Methane Production of Water Buffalo by in Vitro Gas Production Method

作　　者：黄雅莉[1,2] 邹彩霞[1] 韦升菊[1] 梁辛[1] 李舒露[1] 陆天水[3] 杨炳壮[1] 梁贤威[1] 

机构地区：[1]中国农业科学院广西水牛研究所,农业部(广西)水牛遗传繁育重点实验室,南宁530001 [2]广西农业职业技术学院,南宁530007 [3]南京农业大学,南京210095

出　　处：《动物营养学报》2014年第1期126-133,共8页CHINESE JOURNAL OF ANIMAL NUTRITION

基　　金：农业部948项目(2011-G26);广西科学研究与技术开发项目(桂科攻1123005-2;桂科合1010019-24;桂科合2012CA23026);广西自然科学基金项目(2012GXNSFDA053012)

摘　　要：本试验旨在采用体外产气法研究半胱胺对水牛瘤胃发酵参数和甲烷产量的影响。试验采用压力读取式体外产气系统。设计发酵底物的精粗比为32.5∶67.5,半胱胺在精饲料中的添加水平分别为0(对照)、0.2%、0.4%、0.6%、0.8%、1.0%(干物质基础)。结果表明:1)半胱胺对72 h累积产气量影响不显著(P>0.05);可消化有机物含量和代谢能在半胱胺添加水平为0.8%时最高,且0.8%半胱胺组显著高于0.2%、0.4%、0.6%半胱胺组(P<0.05)。2)半胱胺对氨态氮浓度没有显著影响(P>0.05),但0.8%半胱胺组微生物蛋白浓度显著或极显著高于其余各组(P<0.05或P<0.01)。3)乙酸浓度在0.8%半胱胺组最高,与其他各组无显著差异(P>0.05);0.6%半胱胺组较对照组显著增加了乙酸/丙酸(P<0.05)。4)精饲料中添加0.4%以上的半胱胺,可使发酵6、12和24 h甲烷产量显著或极显著减少(P<0.05或P<0.01)。综合上述各项指标可知,体外产气法测定半胱胺添加水平为0.8%时能很好地促进水牛瘤胃微生物发酵和降低甲烷产量。The current experiment was carried out to investigate the effects of cysteamine （Cs） on ruminal fer- mentation parameters and methane production of water buffalo by in vitro gas production method. A reading pressure in vitro gas production system was used. The ratio of concentrate to forage of substrate was 32.5：67.5. Cs was supplemented in concentrate at levels of 0, 0.2%, 0.4%, 0.6%, 0.8% and 1.0% （dry matter basis）, respectively. The results showed as follows： 1 ） Cs had no significant effect on 72 hours accumulation of gas production （P 〉 0.05 ） ; digestible organic matter content and metabolizable energy were the highest in 0.8% Cs group, and significantly higher than those in 0.2%, 0.4% and 0.6% Cs groups （ P 〈 0.05 ）. 2 ） Cs had no significant effect on ammonia nitrogen concentration （ P 〉 0.05 ）, but microbial protein concentration in 0.8% Cs group was significantly higher than that in other groups （P 〈0.05 or P 〈 0.01 ）. 3 ） Acetic acid concentration in 0.8% Cs group was the highest, but had no significant difference with that of other groups （ P 〉 0.05 ） ; compared with the control group, acetic acid/propionic acid was significantly increased in 0.6% Cs group （P 〈0.05）. 4） Cs was supplemented in concentrate at a higher level than 0.4% could significantly decrease methane production after 6, 12 and 24 hours fermentation （P 〈 0.05 or P 〈 0.01 ）. The results of in vitro gas production method indicate that Cs can promote rumen microbe fermenta- tion, but decrease methane production of water buffalo when supplemental level of Cs is 0.8%.

关 键 词：半胱胺 水牛 瘤胃发酵 甲烷 

分 类 号：S823[农业科学—畜牧学]