FOXP1在人卵巢癌细胞系C13和OV2008中的表达及其意义  

Expression and significance of FOXP1 in human ovarian cancer cell lines C13* and OV2008

作　　者：胥琴[1] 刘华[1] 郭恩松[1] 廖菁[1] 朱涛[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院肿瘤生物研究中心,湖北武汉430030

出　　处：《中国妇幼保健》2014年第4期593-595,共3页Maternal and Child Health Care of China

摘　　要：目的:研究FOXP1在人卵巢癌顺铂耐药细胞株C13*和敏感细胞株OV2008的表达和细胞定位情况,并初步探讨其表达意义。方法:①流式细胞术检测不同浓度顺铂处理后两株细胞的凋亡率;②实时荧光定量PCR和免疫荧光技术分别从基因和蛋白水平检测FOXP1在两株细胞中的表达和细胞定位情况。结果:①C13*对顺铂敏感性低于OV2008(P<0.05);②实时荧光定量PCR显示:FOXP1在C13*中的表达明显低于在OV2008中的表达(P<0.05);③免疫荧光显示:FOXP1在两株细胞胞核中均100%表达,而在C13*胞浆中的表达显著低于在OV2008胞浆中的表达(P<0.05),同时大部分OV2008胞浆呈现强"帽子"样染色,而此染色现象在C13*中非常微弱甚至消失。结论:FOXP1在卵巢癌细胞胞浆和胞核中均可表达,而在C13*胞浆的表达下调可能与其耐药有关。Objective： To study the expression and cellular localization of FOXP1 in cisplatin -resistant ovarian cancer cell line C13 and cisplatin -sensitive ovarian cancer cell line 0V2008, and to investigate its significance. Methods： ①Cultured C13 and OV2008 were treated with different concentrations of cisplatin followed by detection of cell apoptosis rate using flow cytometry. ②Real - time fluorescence quantitative PCR and immunofluorescence technique were used to detect the gene and protein expression level of FOXP1 in the two cell lines, respectively. Results： ①Cisplatin sensitivity of C13 was lower than that of OV2008 （P 〈 0. 05 ） .②The results of real - time fluorescence quantitative PCR showed that the gene expression level of FOXP1 in C13 was lower than that in OV2008 （ P 〈 0. 05 ） . ③ The results of immunofluorescence implied that the expression of FOXP1 was found in all nucleus of both C13 and OV2008 cells （ P 〉 0. 05） , but the expression of FOXP1 in cytoplasm of C13 was lower and weaker than that of OV2008 （P 〈0. 05）, and the staining of cy- toplasm in OV2008 looked like the structure of “cap”, but this phenomenon in C13 was very weak. Conclusion： The expression of FOXP1 can be found both in nucleus and cytoplasm of ovarian cancer cell and the down - regulation of FOXP1 in cytoplasm of C13 may be related with drug resistance.

关 键 词：卵巢癌 FOXP1 顺铂 耐药 

分 类 号：R737.31[医药卫生—肿瘤]