几种临床引发Ⅰ型过敏反应的中药注射剂致敏性再评价  

Allergenicity reevaluation of several traditional Chinese medicine injections which can cause the typeⅠhypersensitivity in clinical practice

作　　者：蒋宝平[1,2,3] 田磊[4] 方泰惠[1,2] 程建明[1] 樊晓峰[1] 

机构地区：[1]南京中医药大学 [2]江苏省中药药效与安全性评价重点实验室 [3]江苏省名医验方研究与产业化工程实验室,江苏南京210023 [4]南京中医药大学附属医院,江苏南京210029

出　　处：《安徽医药》2014年第1期20-23,共4页Anhui Medical and Pharmaceutical Journal

基　　金：国家自然科学基金青年基金(No 81102885);国家自然科学基金(No 81173540);江苏省中医药局科技项目(NoLZ11194)

摘　　要：目的通过主动全身过敏试验(ASA)及被动皮肤过敏试验(PCA)对几种临床易于发生Ⅰ型过敏反应的中药注射剂的致敏性进行再评价。方法取生理盐水、卵蛋白、清开灵注射液、生脉注射液、丹参注射液、喜炎平注射液对豚鼠进行主动全身过敏试验和大鼠被动皮肤过敏试验:(1)Elisa法检测主动全身过敏试验中采集的豚鼠血浆中IgE、组胺、类胰蛋白酶、β-氨基己糖苷酶的含量。(2)观察被动皮肤过敏试验中大鼠背部出现的蓝斑情况。结果主动全身过敏试验中卵蛋白组、生脉注射液组、丹参注射液组豚鼠血浆中IgE、组胺及β-氨基己糖苷酶含量均高于生理盐水组;被动皮肤过敏试验中卵蛋白组、生脉注射液组、丹参注射液组可见明显蓝斑。结论主动全身过敏试验结合被动皮肤过敏试验表明卵蛋白、生脉注射液、丹参注射液、清开灵注射液有致敏性,可引起Ⅰ型过敏反应的发生。Objectlve To reevaluate the allergenieity of several Chinese medicine injection which can cause Type I hypersensitivity in clinical praeitee using the active systemic anaphylaxis （ASA） and passive cutaneous anaphylaxis test （PCA）. Methods The guinea pig active systemic anaphylaxis test and the rat-homologous passive cutaneous anaphylaxis test were carried out on the normal saline group,ovalbumin group,Qingkailing injection,Shengmai injection,Danshen injection group and Xiyanping injection group. The contents of igE,histamine,t^Tptase and 13-hexosaminidase in plasma of guinea pigs were determined by ELISA assay. Whether there was a blue spot in the back of PCA rats was observed. Results in the ASA test,the contents of igE,histamine and 13-hexosaminidase in plasma of guinea pigs of owlbumin group, Shengmai injection group and Danshen injection group were higher than that in the normal saline group. in the passive cutaneous anaphylaxis test,ovalbumin group,Shengmai injection group and Danshen injection group showed obvious blue spots. Conelualons 0wlbumin,Shengmai injection,Danshen injection and Qingkailing injection can cause the occurrence of type I hypersensitivity reaction.

关 键 词：Ⅰ型过敏反应 致敏性 ELISA 

分 类 号：R286[医药卫生—中药学]