ALDH1A1基因表达沉默对鼻咽癌细胞的增殖抑制作用  

ALDH1A1 downregulation inhibits cell growth and proliferation in nasopharyngeal carcinoma cells

作　　者：吴爱兵[1] 邓旭斌[2] 杨志雄[1] 沈湘[1] 李姝君[1] 黎明春[1] 吴昆鹏[1] 

机构地区：[1]广东医学院附属医院肿瘤中心,广东湛江524000 [2]南方医科大学肿瘤研究所,广东广州515000

出　　处：《现代肿瘤医学》2014年第2期269-272,共4页Journal of Modern Oncology

基　　金：国家青年科学基金资助项目(编号:81201672);湛江市科技攻关计划项目(编号:2012C3104032)

摘　　要：目的:探讨干扰乙醛脱氢酶1A1(ALDH1A1)基因表达后对鼻咽癌5-8F和CNE2细胞生长、增殖的影响。方法:构建ALDH1A1 siRNA表达质粒稳定转染鼻咽癌5-8F和CNE2细胞,Western blot检测干扰ALDH1A1基因蛋白表达水平。MTT、平板克隆、裸鼠成瘤实验检测干扰ALDH1A1基因前后鼻咽癌5-8F和CNE2细胞的增殖、克隆及成瘤能力。结果:与空白组和阴性对照组相比,干扰ALDH1A1表达后5-8F和CNE2细胞增殖、克隆及肿瘤形成能力明显受到抑制。结论:干扰ALDH1A1基因表达,可抑制鼻咽癌细胞的生长、增殖、克隆及成瘤能力。Objective：To explore the growth and proliferation of nasopharyngeal carcinoma （NPC） 5-8F and CEN2 cell lines by the expression of suppressed ALDH1A1 gene.Methods：ALDH1A1 specific siRNA expression plasmid and non-specific siRNA expression plasmid were constructed and transected in 5-8F and CNE2 cells.The protein level of ALDH1A1 was assessed by Western blot.We assayed the proliferation and tumorigenesis of ALDH1A1 in vitro and vivo.Results：5-8F and CNE2 cells grew slower and had lower clone formation efficiency and had lower tumorigenesis than control and untreated cells after suppressed ALDH1A1 gene in vitro and vivo.Conclusion：ALDH1A1 downregulation inhibits cell proliferation,clone formation efficiency and tumorigenesis in nasopharyngeal carcinoma cells.

关 键 词：鼻咽癌 乙醛脱氢酶1A1 SIRNA 增殖 

分 类 号：R73-362[医药卫生—肿瘤] R739.63[医药卫生—临床医学]