PKM2 siRNA对人甲状腺乳头状癌细胞生长和增殖的影响  

Effect of PKM2 siRNA on growth and proliferation of papillary thyroid cancer cell

作　　者：刘睿[1] 王晓楠[2] 张薇[3] 关海霞[1] 

机构地区：[1]中国医科大学附属第一医院内分泌科,内分泌研究所,辽宁省重点实验室,辽宁沈阳110001 [2]中国医科大学附属第一医院老年病科,辽宁沈阳110001 [3]中国医科大学附属盛京医院内分泌科,辽宁沈阳110011

出　　处：《现代肿瘤医学》2014年第2期278-282,共5页Journal of Modern Oncology

基　　金：国家自然科学基金(编号:81200246)

摘　　要：目的:研究PKM2在甲状腺乳头状癌(PTC)中的表达特性及其在PTC中的作用。方法:用抗PKM2的小干扰RNA转染PTC细胞。Western blot和RT-PCR技术分析细胞中PKM2的表达。MTT法和细胞平板克隆形成实验评估细胞的生长和增殖。采用乳酸、ATP和葡萄糖试剂盒分别测定细胞代谢产生的乳酸、ATP及消耗的葡萄糖。结果:PKM2在PTC中异常高表达。在PTC细胞系中应用特异性抗PKM2 siRNA可延缓细胞生长并降低其克隆形成能力。PKM2 siRNA可降低PTC细胞乳酸、ATP的产生,减少葡萄糖的消耗。结论:PKM2高表达可通过激活糖酵解的方式使PTC细胞具有选择性生长优势。PKM2异常高表达可能作为新的生物标记物,并成为PTC的潜在治疗靶点。Objective：To investigate the expression pattern of PKM2 in papillary thyroid cancer（PTC） and the possible role of PKM2 in PTC.Methods：PTC cells were transfected with small interfering RNA against PKM2.PKM2 expression in cells was analyzed by Western blot and RT-PCR.Cell growth and proliferation was evaluated by MTT assays and colony formation assays.Lactate and ATP production and glucose consumption by cells were determined using commercial assay kits.Results：PKM2 was aberrantly overexpressed in PTC.Specific siRNA against PKM2 in PTC cell lines retarded cell growth and decreased their colony-forming capability.PKM2 knockdown also reduced lactate and ATP production,and glucose consumption by PTC cells.Conclusion：Overexpression of PKM2 provides a selective growth advantage for PTC cells through activation of glycolysis.Aberrant PKM2 overexpression may serve as a novel biomarker and a potential treatment target for PTC.

关 键 词：甲状腺乳头状癌 PKM2 RNA干扰 糖酵解 

分 类 号：R736.1[医药卫生—肿瘤]