毛细管区带电泳同时分离测定四种多酚类化合物  

Separation and determination of four kinds of polyphenolic compounds by capillary zone electrophoresis

作　　者：刘光辉[1] 李菲[1] 胡旭芳[1] 邵娅婷 曹秋娥[1] 

机构地区：[1]云南大学化学科学与工程学院,教育部自然资源药物化学重点实验室,昆明650091

出　　处：《分析试验室》2014年第2期191-194,共4页Chinese Journal of Analysis Laboratory

基　　金：教育部博士点专项基金(20125301110005)资助

摘　　要：在由1．0mmol／Lβ-环糊精、乙二醇（1＋9）和10mmol／L硼砂组成的运行缓冲溶液（pH9．11）中，采用22kV的分离电压、25℃的毛细管柱温、200nm的检测波长和5．0S的压力（3450Pa）进样时间，建立了可以在21min内同时分离测定儿茶素、表儿茶素、原儿茶醛及原儿茶酸的高效毛细管电泳方法。检出限（S／N=3）依次为0．27、O．18、0．52和0．41μg／mL。方法用于普洱茶、丹参注射液和中药儿茶与金荞麦片中这4种组分的测定，相对标准偏差在4％以内，加标回收率在96．1％-105．4％之间。A new capillary zone electrophoresis method for separation and determination of catechin （C）, epicatechin （ EC）, protocatechuic aldehyde （PAD） and protocatechuic acid （PAC） has been developed. Four kinds of polyphenolic compounds could be effectively separated and determined within 21 min in a running buffer （pH9. 11 ） consisting of 10 mmol/L Na2B4OT, 1.0 mmol/L β-CD and 10. 0% （V/V） ethylene glycol with an applied voltage of 22 kV, capillary temperature of 25 ℃ injection of 5 s at 3.45 kPa and a detection wavelength of 200 nm. The detection limits （S/N = 3 ） for catechin, epicatechin, protocatechuic aldehyde and protocatechuic acid were 0. 27, 0. 18, 0. 52and 0.41 μg/mL, respectively. The proposed method has been successfully used for the determination of the four constituents in puer tea, danshen injections, Catechu and Jinqiaomai tablets with the relative standard deviation of below 4. 0% and recoveries between 96. 1% and 105.4%.

关 键 词：毛细管区带电泳 儿茶素 表儿茶素 原儿茶醛 原儿茶酸 

分 类 号：O657.8[理学—分析化学]