冬凌草甲素对C6大鼠脑胶质瘤裸鼠异种移植模型的抗肿瘤疗效研究  

Anti-tumor Efficacy of Oridonin to Rat C6 Glioma in Nude Mouse Xenograft Model

作　　者：尹波[1] 李志伟[1] 张弩[1] 盛汉松[1] 林坚[1] 

机构地区：[1]温州医科大学附属第二医院,浙江温州325000

出　　处：《肿瘤学杂志》2014年第1期34-39,共6页Journal of Chinese Oncology

基　　金：温州市科技局科研基金资助项目(Y20090282)

摘　　要：[目的]利用C6大鼠脑胶质瘤裸鼠异种移植模型研究冬凌草甲素对大鼠C6脑胶质瘤增殖的抑制效果。[方法]应用C6大鼠脑胶质瘤细胞株建立了该肿瘤的BALB/C裸鼠异种移植模型,腹腔注射低、中、高3个剂量的冬凌草甲素和固定剂量的对照化疗药物卡氮芥,比较给药前后的相对肿瘤增殖率、肿瘤抑制率和肿瘤体积变化,单因素方差分析比较各组间差异。[结果]在给药后的22d,冬凌草甲素低、中、高3个剂量组所对应动物模型的相对肿瘤增殖率均小于100%,且随着冬凌草甲素给药剂量的增加而降低;肿瘤抑制率均为正值,且随着给药剂量的增加而升高,但3个剂量组之间差异无统计学意义(F=7.59,P>0.05);在给药后的22d内,未给药组和所有给药组动物模型体内的肿瘤体积均呈逐日增大的趋势,但在12d后,高剂量冬凌草甲素给药组动物模型的肿瘤体积增量要显著小于未给药组(F=41.15,P<0.05),在给药组中,随着给药剂量的增加,在同一天动物模型所对应的肿瘤体积增量呈现递减趋势。[结论]冬凌草甲素对大鼠C6脑胶质瘤的增殖具有一定的抑制效果。[Purpose] To study the inhibitory effect of oridonin on proliferation of rat C6 glioma in nude mouse xenograft model with rat C6 glioma. EMethods] BALB/C nude mouse xenograft models with rat C6 glioma were built by rat C6 glioma cell line.Low,medium and high dosages of oridonin, and fixed-dosages carmustine as control were consecutively administrated for nine days intraperitoneally. Tumor proliferation,inhibition rate and volume change among different groups were compared after administration.Experimental data were statistically analyzed by One-Way ANOVA. [Results] Tumor proliferation rates in the 3 different oridonin dosages groups were all less than 100% and as the oridonin dosages increasing the tumor proliferation rate decreasing.Tumor inhibitory rates were all increase as administration dosage increase,but there was no significant difference （F=7.59 ,P〉0.05）.After 22 days＇ administration,tumor vol- umes of the administrated and non-administrated animal models all showed increase day by day.After 12 days＇ administration ,the tumor volume increment in models with oridonin was sig- nificantly less than that without oridonin（F=41.15 ,P〈0.05）.The tumor volume increment showed decline trend on the same day as administration dosage increased. [Conclusion] Oridonin has inhlhitorv effect on nroliferation of rat C6 ~lioma.

关 键 词：冬凌草甲素 卡氮芥 胶质瘤 异种移植 

分 类 号：R739.41[医药卫生—肿瘤]