miR-155在直肠癌细胞炎性条件下的表达  

Effect of miR-155 expression and cell proliferation in rectal cancer cell lines

作　　者：何培生[1] 刘作金[2] 贾尚琼[3] 郑康霞[1] 李冠华[1] 刘磊[2] 黄臻[2] 

机构地区：[1]重庆三峡中心医院普外二科,重庆404100 [2]重庆医科大学附属第二医院肝胆外科 [3]重庆三峡中心医院骨科

出　　处：《胃肠病学和肝病学杂志》2014年第1期32-35,共4页Chinese Journal of Gastroenterology and Hepatology

摘　　要：目的研究在直肠癌细胞炎性条件下的miR-155表达。方法通过研究直肠癌细胞中各种炎性条件下0 h、1 h、4 h、8 h、12 h、24 h的时间段细胞计数,运用统计学绘制细胞生长曲线,利用流式细胞仪检测24 h内的细胞周期变化情况。结果与未加入慢性炎症应激药物比较,H2O2、SPER/NO、HU作用于直肠癌细胞后,直肠癌细胞中miR-155表达量均升高,差异有统计学意义(P<0.05),但在不同药物及不同细胞之间miR-155表达差异无统计学意义(P>0.05)。不同药物作用后,miR-155表达出现峰值的时间不同。结论 miRNA具有癌基因或抑癌基因的功能,其表达异常后与多种人类癌症发生相关,在肿瘤发生、发展过程中有重要作用。Objective To study the effects of miR-155 expression and cell proliferation in rectal cancer cell lines. Methods Cells count of 0 h, 1 h, 4 h, 8 h, 12 h, 24 h under various inflammation conditions were studies. Cell growth curve was detected by flow cytometry in 24 h cell cycles. Results Compared with non medicated chronic inflam- matory stress, the expression of miR-155 were increased in rectal cancer cells after H202, SPER/NO, HU, the differ- ence was statistically significant （P 〈 0.05） , but miR-155 expression had no significant difference in the different drugs and different cells （P 〉0.05）. After treated with different drugs, the peak time of miR-155 expression was different. Conclusion miRNA can play the function of oncogenes or tumor suppressor genes, abnormal expression of a variety of human cancers, tumors, development of important factors.

关 键 词：MIRNA 直肠癌 细胞周期 

分 类 号：R735.37[医药卫生—肿瘤]