样品制备过程对测定水中溶解态微囊藻毒素的影响  

THE INFLUENCES OF SAMPLE PREPARATION PROCESSES ON THE DETERMINATION OF DISSOLVED MICROCYSTINS IN FRESHWATERS

作　　者：贾云璐[1,2] 陈伟[1] 赵爽[1,2] 周起超[1,2] 宋立荣[1] 

机构地区：[1]中国科学院水生生物研究所淡水生态与生物技术国家重点实验室,武汉430072 [2]中国科学院大学,北京100049

出　　处：《水生生物学报》2014年第1期87-91,共5页Acta Hydrobiologica Sinica

基　　金：国家高技术研究发展计划(863计划)项目课题(编号:2009AA063005);国家自然科学基金项目(编号:40971249)资助

摘　　要：系统比较了5种不同材质滤膜对于制备溶解态微囊藻毒素的影响,发现了影响藻毒素测定样品前处理的关键操作步骤。结果表明,醋酸纤维滤膜(CA filter)最多可吸附样品中79%的藻毒素,导致测得的MCs浓度严重偏低。玻璃纤维(GF/C)滤膜和聚醚砜(PES)材质滤器对制备溶解态微囊藻毒素过程影响很小。另外,发现离心法无法完全去除野外水华样品中藻细胞,反而可能导致藻细胞破裂,释放藻毒素,影响水中溶解态微囊藻毒素的测定。研究结果将对发展水体中溶解态藻毒素测定标准方法提供依据。Several processing protocols were systematically investigated for the preparation of dissolved microcystins （MCs） samples. Some key parameters affecting toxin determinations in sample preparation process were further identi-fied and quantified. We evaluate the influence of five different filters on the preparation of MCs samples. Results indi-cated that nearly 79% of added MCs could be absorbed by some membranes like CA filter for the filtration, which lead to the significant underestimation of MCs risks associated with toxin contaminations. Meanwhile, GF/C filter and PES filter tip are suitable for the preparation of dissolved MCs samples. In addition, centrifugation with the aim of removing algal cells also has significant impact on dissolved MCs determination. Results from this study will provide important information on the determination of dissolved microcystins and further lead to the standardization of the extracellular toxin determinations in risk assessments.

关 键 词：溶解态微囊藻毒素 滤膜 测定 样品制备 

分 类 号：X172[环境科学与工程—环境科学] Q-337[生物学]