S100A7对食管鳞癌细胞增殖及侵袭能力的影响及可能机制  

Effects of S100A7 on the proliferation and invasiveness of esophageal squamous cell carcinoma EC9706 cells and possible mechanisms

作　　者：曲蕴慧[1] 刘红涛[3] 李晟磊[2] 

机构地区：[1]郑州大学第一附属医院检验科,450052 [2]郑州大学第一附属医院病理科,450052 [3]郑州大学生命科学学院细胞生物学研究室

出　　处：《中华医学杂志》2014年第2期144-147,共4页National Medical Journal of China

基　　金：河南省教育厅自然科学基金（2010A310008）

摘　　要：目的探讨S100A7在食管鳞癌组织中的表达及其对食管鳞癌EC9706细胞增殖和侵袭能力的影响。方法利用原位杂交及免疫组织化学法检测食管鳞癌组织中S100A7mRNA和蛋白的表达，以S100A7siRNA转染食管鳞癌EC9706细胞，利用实时荧光定量PCR和Western印迹技术检测$100A7mRNA及蛋白的表达，并利用细胞计数试剂盒8（CCK-8）试剂及Boyden小室分别检测S100A7表达对细胞增殖及侵袭能力的影响，用Western印迹技术分析S100A7表达对基质金属蛋白酶2（MMP-2）表达的影响。结果食管鳞癌组织中S100A7mRNA及蛋白表达阳性率分别为72．0％和69．3％，显著高于正常食管黏膜组织的10．7％和8．0％（妒=58．174和59．483，均P：0．000）。S100A7siRNA组S100A7mRNA及蛋白表达均显著低于未处理组和对照siRNA组（0．235±0．056比I和0．921±0．038及0．119±0．025比0．518±0．129和0．481±0．130：F=346．307，P=0．000及F=12．826，P=0．007）。S100A7表达下调能明显抑制食管鳞癌EC9706细胞的增殖和侵袭，并伴有MMP-2蛋白表达的下调。结论S100A7高表达可能在食管鳞癌的发生发展中有重要作用，其表达下调对食管鳞癌细胞增殖及侵袭能力的抑制作用可能与MMP一2表诀下调密切相关。Objective To explore the expression of S100A7 in esophageal squamous cell carcinoma （ESCC） and examine the effect of S100A7 down-regulated expression mediated via RNA interfering on cell proliferation and invasion. Methods The expressions of S100A7 mRNA and protein were analyzed by in situ hybridization and immunohistochemistry. S100A7 siRNA was transfected into ESCC cell line EC9706 and the expressions of S100A7 mRNA and protein were detected by real-time polymerase chain reaction （PCR） and Western blot. Furthermore, the effects of down-regulated of SIOOA7 on cell proliferation and invasion were examine by Cell Counting Kit-8 （CCK-8） and Boyden chamber respectively. Finally the effect of down- regulated expression of S100A7 on matrix metalloproteinase-2 （MMP-2） protein was analyzed by Western blot. Results Positive expression ratios of S100A7 mRNA and protein expressions in ESCC tissues （72. 0% and 69. 3% ） were significantly higher than those in normal esophageal tissues （10. 7% and 8.0% ） （X^2 = 58. 174 and 59. 483, both P = 0. 000）. The expressions of S100A7 mRNA and protein in S100A7 siRNA group were markedly lower than those in untreated group （mRNA： 0. 235 ± 0. 056 vs 1, protein ： 0. 119 ± 0. 025 vs 0. 518 ± 0. 129, both P 〈 0.05 ）. Additionally, the down-regulated expression of S100A7 resulted in the proliferation inhibition and the decreased invasiveness in EC9706 cells. And it was coupled with a down-regulation of MMP-2 protein. Conclusion S100A7 may play an essential role in the occurrence and development of ESCC and the decreased invasiveness of EC9706 cells mediated by the down- regulated expression of S100A7 may be closely associated with the down-regulation of MMP-2 protein.

关 键 词：食管肿瘤 癌 鳞状细胞 S100蛋白质类 细胞增殖 肿瘤侵袭 

分 类 号：R735.1[医药卫生—肿瘤]