慢性淋巴细胞白血病患者骨髓中CD200表达及与预后的关系  

Expression of CD200 in the bone marrow of chronic lymphocytic leukemia patients and its correlations with clinical prognosis

作　　者：王晓娜[1] 张正昊[1] 刘洋[1] 王蕾[1] 袁海龙[1] 谢平[1] 曲建华[1] 

机构地区：[1]新疆医科大学第一附属医院血液科,新疆乌鲁木齐830054

出　　处：《细胞与分子免疫学杂志》2014年第1期75-78,共4页Chinese Journal of Cellular and Molecular Immunology

基　　金：新疆维吾尔自治区自然科学基金(2013211A100)

摘　　要：目的初步探讨慢性淋巴细胞性白血病(CLL)患者骨髓中CD200的表达及其与免疫表型、染色体核型的关系和与预后相关性。方法采用流式细胞术检测40例初诊CLL患者骨髓中CD200的数量。以CD19+CD200+表达低于50%为CD200low组(18例),大于或等于50%的为CD200high组(22例)。结果 CD200low组(18例)年龄、男性人数、初次白细胞(WBC)数量、初次淋巴细胞(LY)数量、初次LY%、淋巴结发生率显著低于CD200high组(22例)(P<0.05);CD200low组初次血红蛋白(HB)量高于CD200high组(P<0.05)。CD200low组CD19+、CD19+CD23+、CD19+CD160+抗原表达的平均百分数及Zeta链相关蛋白-70(ZAP-70)阳性率明显低于CD200high组(P<0.05)。Binet分期中,CD200low组A期概率多于CD200high组,CD200high组大部分处于C期,差异有统计学意义(P<0.05)。Rai分期中,CD200low组患者大部分处于Ⅰ期,CD200high组大部分处于Ⅲ期,差异有统计学意义(P<0.05)。结论 CD200对诊断疾病、预后判断、实施个体化治疗、延长患者生存期等具有重要意义。Objective To explore the expression of CD200 in the bone marrow of chronic lymphocytic leukemia （CLL） patients and its correlations with clinical features, phenotype, chromosome type and prognosis. Methods 40 patients with CLL were tested before chemotherapy. The CD200 in the bone marrow samples were detected by the flow cytometry. The expression of CD19 ＋ CD200 ＋was less than 50% in 18 patients, which were assigned into CD200^（low）group, and the expression was more than or equal to 50% in 22 patients, which were in CD200h＊~h group. Results Compared with the CD200high group, the CD200^（low） group had lower mean age, fewer men, lower initial WBC count, lower initial lymphocyte count, lower initial percentage of lymphocyte, lower lymph node incidence and higher initial hemoglobin （P 〈 0.05）. The average percentage of CD19＋, CD19＋ CD23 ＋ , CD19 ＋ CD160 ＋ and the positive rate of Zeta chain-associated protein 70 （ZAP-70） in the CD200^（low） group were significantly lower than those in the CD200high group （P〈0.05）. In Binet stage classification, the patients of the CD200^（low） group were mostly in phase A, and the ones of the CD200high group were mostly in phase C （P〈0.05）. In Rai stage classification, the patients of the CD200^（low） group were mostly in phase I, and the ones of the CD200high group were mostly in phase III stage （ P 〈 0.05）. Conclusion CD200 might be very important for the diagnosis, prognosis, individualized treatment and the longer survival time of CLL patients.

关 键 词：慢性淋巴细胞性白血病 CD200 Zeta链相关蛋白-70 IgV_(H) 

分 类 号：R733.72[医药卫生—肿瘤] R392.11[医药卫生—临床医学]