玉米穗行数全基因组关联分析  

Genome-wide Association Analysis of Kernel Row Number in Maize

作　　者：张焕欣[1] 翁建峰[1] 张晓聪[1] 刘昌林[1] 雍洪军[1] 郝转芳[1] 李新海[1] 

机构地区：[1]中国农业科学院作物科学研究所/作物分子育种国家工程实验室,北京100081

出　　处：《作物学报》2014年第1期1-6,共6页Acta Agronomica Sinica

基　　金：国家自然科学基金项目(31201219);国家重点基础研究发展计划(973计划)项目(2011CB100106)资助

摘　　要：穗行数是玉米产量的重要组成性状,其遗传解析对高产育种具有指导意义。本文以203份主要玉米自交系为材料,2007年在新疆乌鲁木齐、吉林公主岭和海南三亚进行穗行数测定;采用分布于玉米基因组的41 101个单核苷酸多态性(SNP)标记对穗行数进行关联分析。共鉴定出9个与穗行数显著关联(P<0.0001)的SNP,分别位于染色体框1.02、1.10、7.03、8.02、9.06和10.03。8个SNP位于已定位的数量性状座位(QTL)区间内。在显著SNP位点LD区域内发掘出4个候选基因,分别编码含F-box结构域的生长素受体蛋白、玉米kn1蛋白、AP2结构域蛋白和富亮氨酸重复的跨膜蛋白激酶。采用全基因组关联分析策略发掘穗行数基因位点及候选基因,将为克隆控制玉米产量性状基因奠定基础。Kernel row number（KRN） is one of grain yield components in maize（Zea mays L.）.Investigation of its genetic architecture will help develop high-yield varieties in maize.In this study,the KRN in a panel of 203 maize inbred lines was detected in Urumqi of Xinjiang,Gongzhuling of Jilin,and Sanya of Hainan in 2007,and used to perform the genome-wide analysis for KRN using MaizeSNP50 BeadChip.A total of nine SNPs were found to be significantly associated with KRN at a threshold of P 0.0001,which were on chromosome Bins 1.02,1.10,7.03,8.02,9.06,and 10.03,respectively.Eight of these SNPs were located in the QTL intervals reported previously.Meanwhile,four candidate genes were scanned,encoding auxin signaling F-box containing protein,kn1 protein,AP2 domain containing protein and leucine-rich repeat transmembrane protein kinase respectively.In summary,these identified genes and SNPs will offer essential information for cloning yield-related genes in maize.

关 键 词：玉米 穗行数 全基因组关联分析 候选基因 

分 类 号：S513[农业科学—作物学]