两个水稻叶片反向卷曲突变体的表型及遗传分析  

Phenotypic and genetic analysis of two reverse rolled leaf mutants

作　　者：申奥[1,2] 苏余燕 宋远丽[1,2] 栾维江[1,2] 

机构地区：[1]天津师范大学生命科学学院,天津300387 [2]天津师范大学天津市动植物抗性重点实验室,天津300387

出　　处：《天津师范大学学报（自然科学版）》2014年第1期67-71,共5页Journal of Tianjin Normal University：Natural Science Edition

基　　金：国家自然科学基金资助项目(31171515);天津市自然科学基金重点资助项目(11JCZDJC17900)

摘　　要：为了揭示水稻叶片卷曲的形态建成,分析了水稻Ac/Ds转座子插入突变体库中的2个叶片反向卷曲突变体的表型及组织形态.研究发现,与野生型平展叶片相比,内卷突变体和外卷突变体叶片中泡状细胞数目明显减少,这可能是造成水稻叶片卷曲的重要原因.另外,内卷突变体叶片主脉薄壁细胞少,薄壁细胞崩裂形成的气腔面积较大.对2个卷叶突变体的纤维素含量进行测定发现,内卷突变体叶片及茎杆中纤维素含量明显减少,而外卷突变体叶片及茎杆的纤维素含量与野生型没有显著差异,说明2个卷叶突变体叶片的卷曲可能由不同基因突变造成.分析了2个水稻卷叶突变体的遗传规律,结果表明,二者均由隐性单基因控制.To investigate the architecture of the rolled leaf morphology in rice, the features of phenotype and histological level in 2 reverse rolled leaf mutants derived from rice Ac/Ds transposons insertional mutant library were investingated. The results showed that the number of bulliform cells was reduced in 2 reverse rolled leaf mutants fAd-mutant： adaxial rolled mutant; Ab-mutant： abaxial rolled mutant） compared with the normal flat leaf in wild type, which may be an important cause of the rolled leaf in rice. And the number of parenchyma also was reduced and dehisced to 2 large air cavities in A b- mutant. The cellulose content of leaf and culm in 2 rolled leaf mutant and wild type was measured, and the result showed that the cellulose content of leaf and culm in Ab-mutant was significant reduced compared with Ad-mutant and wild type. But the cellulose content of leaf and culm in A d-mutant was not significantly different from that of wild type. This result suggested that Ab-mutant and Ad-mutant may be different in mutation of gene loci. In addition, the genetic rule of Ab- mutant and A d-mutant was analyzed, and the result showed that both of them were controlled by single recessive gene.

关 键 词：水稻 叶片卷曲 表型分析 遗传分析 

分 类 号：Q943.2[生物学—植物学]