N-(4-羟基苯基)维生素甲酰胺抑制人肺腺癌A549细胞体外迁移机制  

Mechanism of N-(4-hydroxyphenyl) retinoide in inhibiting the migration of human lung adenocarcinoma of A549 cells

作　　者：张玲[1] 黄道斌[2] 吕俊[1] 汪渊[3] 

机构地区：[1]皖南医学院生化教研室,芜湖241002 [2]皖南医学院计算机教研室,芜湖241002 [3]安徽医科大学分子生物学实验室、生物化学与分子生物学教研室,安徽省/省部共建教育部重要遗传病基因资源利用重点实验室,合肥230032

出　　处：《安徽医科大学学报》2014年第2期137-140,共4页Acta Universitatis Medicinalis Anhui

基　　金：安徽省高校自然科学基金(编号:KJ2010B245)

摘　　要：目的研究N-(4-羟基苯基)维生素甲酰胺(4-HPR)对人肺腺癌细胞A549体外迁移能力的影响及其作用机制。方法 1μmol/L全反式维甲酸(ATRA)和4-HPR处理肺腺癌A549细胞24 h后,细胞划痕实验检测其对A549细胞体外迁移能力的影响;Western blot法检测骨桥蛋白(OPN)、肌球蛋白轻链激酶(MLCK)的表达和肌球蛋白轻链(MLC)磷酸化程度;细胞划痕实验观察MLCK抑制剂ML-7对A549细胞迁移能力的影响。结果 1μmol/L 4-HPR处理的A549细胞与细胞对照以及溶剂对照细胞相比,迁移距离明显降低(P<0.05);Western blot分析结果表明1μmol/L 4-HPR可明显降低A549细胞MLCK的表达和MLC磷酸化(P<0.05),而对OPN表达无显著影响;ML-7可明显减少A549细胞迁移距离(P<0.05)。结论 4-HPR可能通过减少MLCK的表达和MLC磷酸化,抑制A549细胞的体外迁移。Objective To investigate the influences of N-（4-hydroxyphenyl）retinoide （4-HPR） on the migration of human lung adenocarcinoma cells and its mechanism. Methods Cell scarification test was performed to measure the migration of A549 cells treated with 1 μmoL/L 4-HPR. The expression level of osteopontin （OPN） , myosin light chain kinase （MLCK） and phosphorylation of myosin light chain（MLC） in A549 cells treated by 4-HPR was detec- ted by Western blot, respectively. The effect of ML-7, a selective inhibitor of MLCK, on the migration of A549 cells was analyzed by cell scarification test. Results Compared with cell and solvent control group, the migration distance of A549 cells treated with 1 ixmoL/L 4-HPR was obviously decreased（ P 〈 0.05 ）. The Western blot analy- sis showed that 1 μmoL/L 4-HPR could significantly reduce the expression of MLCK and phosphorylation of MLC protein （ P 〈 0.05 ） , while had no influence on the expression of OPN. ML-7 could decrease the distances of migra- tion of A549 cells notably （P 〈 0.05）. Conclusion 4-HPR may inhibit the migration of A549 cells through de- creasing the expression of MLCK and phosphorylation of MLC protein.

关 键 词：4-HPR 人肺腺癌A549 迁移 肌球蛋白轻链激酶 细胞划痕实验 

分 类 号：R734.2[医药卫生—肿瘤] R329.24[医药卫生—临床医学]