免疫金纳米笼对人乳腺癌细胞靶向光热治疗作用  

Immuno gold nanocages on human breast cancer cells targeted photothermal therapy

作　　者：田开亮[1] 朱立新[1] 许小亮[2] 尹乃强 黄乾鹏 赵红川[1] 耿小平[1] 

机构地区：[1]安徽医科大学第一附属医院普通外科,合肥230022 [2]中国科学技术大学物理系纳米功能材料实验室,合肥230026

出　　处：《安徽医科大学学报》2014年第2期164-167,共4页Acta Universitatis Medicinalis Anhui

基　　金：国家自然科学基金(编号:51272246;81172082)

摘　　要：目的基于金纳米笼的光热转换特性,观察经抗体修饰的免疫金纳米笼对人乳腺癌细胞MCF-7的靶向光热效应,探讨其对肿瘤细胞的杀伤效果及其细胞毒性。方法由银(Ag)立方纳米晶与次氯金酸(HAuCl4)置换反应得到金纳米笼,经Anti-CK19抗体修饰后制成具有生物靶向性的免疫金纳米笼。实验组(A组):将金纳米笼-抗体探针链接细胞后,由功率密度为1.5 W/cm2、波长为808 nm的激光照射30min;对照组(B组)分为2组:BⅠ组经相同光照强度和波长的激光单纯光照30 min;BⅡ组为单纯加金纳米笼无光照。观察A、B组温度随时间变化情况,通过荧光终止剂细胞染色及流式细胞术检测免疫金纳米笼的光热杀伤效应,应用四甲基偶氮唑蓝(MTT)比色法观察其细胞毒性。结果 A组温度升高明显,达48℃,而B组温度无明显变化,维持在35℃左右。荧光终止剂细胞染色及流式细胞术显示A组细胞大量凋亡,而B组细胞活性基本未受影响(P<0.05)。MMT法显示细胞毒性较小(P<0.05)。结论免疫金纳米笼对MCF-7细胞具有靶向杀伤作用,被用于光热治疗。Objective Based on the solar-thermal conversion characteristics of the gold nanocages, we investigated the photothermal effect and ceel toxicity of immune gold nanocage in a human breast cancer cell MCF-7 . Methods Gold nanocages were obtained through the replacement reaction between silver （ Ag ） cubic nanocrystalline and chloroauric acid（ HAuCl4 ） . Then the nanoparticles were modified with anti-CK19 antibody to have gold biological targeting characteristics. In experimental group, gold nanocage-antibody probe was applied to the cell, and then treated with the power density 1. 5 W/cm2 , the wavelength of 808 nm laser for 30 min; in control group, gold nanocage-antibody probe was applied to the cell, but no light treatment, or cells were treated with light only. At the end of each treatment, MCF-7 cells were collected for fluorescence termination agent cell staining and flow cytome-try assay for the photothermal effect. The cytotoxicity was examined by colorimetric method of methyl thiazolyl tet-razolium（ MTT） . Results The temperature increased significantly to 48 ℃ in control group, but not in control group （ remained 35 ℃） . Cells in experimental group showed significant apoptosis in fluorescent termination agent cell staining and flow cytometry assay, while the cells in control group showed no apoptosis（P＆lt;0.05）. MMT ex-periments showed that cytotoxicity of this method was small（P＆lt;0. 05）. Conclusion Immuno gold nanocages have a specific targeting killing effect on MCF-7 cell, and be used for photothermal therapy.

关 键 词：光热治疗 金纳米笼 乳腺肿瘤 治疗 

分 类 号：R737.9[医药卫生—肿瘤] R456.1[医药卫生—临床医学]