武当木兰种群遗传结构的ISSR分析  

Analysis of Genetic Structure of Magnolia sprengeri Populations Based on ISSR Markers

作　　者：杨梅[1] 张敏[1] 师守国[2] 康永祥[1] 刘建军[1] 

机构地区：[1]西北农林科技大学,杨凌712100 [2]运城学院,运城044000

出　　处：《林业科学》2014年第1期76-81,共6页Scientia Silvae Sinicae

基　　金：国家林业局公益性行业科研专项(200904004)

摘　　要：采用ISSR分子标记对湖北、陕西及重庆等地区9个典型武当木兰种群的遗传多样性和遗传结构进行研究。用8条引物共检测到207个有效位点,其中多态性位点195个。在物种水平上,多态位点百分率(PPB)为94.20%,Nei遗传多样性指数(H)和Shannon信息指数(I)分别为0.171 9和0.288 2;种群水平的PPB,H和I分别为43.64%,0.128 4和0.198 8。分子变异分析(AMOVA)显示,武当木兰种群内变异占总变异的68.10%(P<0.001),种群间的占31.90%(P<0.001),种群间的基因分化系数(G ST)为0.257 0,说明武当木兰种群内和种群间均存在着显著的遗传分化,但种群内变异对总变异的贡献较大。种群间的基因流(N m)为1.445 6,9个种群的平均遗传距离为0.040 0。算术加权平均数法(UPGMA)将9个武当木兰种群分为3大类群,聚类结果与大多数种群的地理分布及花部特征的划分大致吻合。Magnolia sprengeri is an important ornamental plant species.In this study 8 ISSR primers were used to examine the genetic diversity and genetic structure of 9 natural M.sprengeri populations which included 71 individuals,collected from Shaanxi,Hubei,Chongqing of China which belong to the main distribution area.A total of 207 valid loci were obtained,of which 195 were exhibited polymorphism.The percentage of polymorphic bands （PPB） were 94.20% at species level and 43.64% at population level,and the mean diversity estimated with Nei＇ s index （H） and Shannon＇ s information index （Ⅰ） was 0.171 9 and 0.288 2 at species level,and 0.128 4 and 0.198 8 at population level,respectively.Analysis of molecular variance （AMOVA） demonstrated that the proportion of genetic variation was 68.10% （P 〈 0.001） within population,and 31.90% （P 〈 0.001） among populations.The differential index （GsT） among the populations was 0.257 0.All these results indicated that there was a significant genetic differentiation both within and among the populations.The gene flow （Nm） among populations was 1.445 6 and the genetic distance among 9 populations was 0.040 0.The UPGMA cluster analysis divided the 9 populations into 3 major groups,which was consistent well with the geographical distribution and floral characters of most of the populations except for some transitional populations.

关 键 词：武当木兰 ISSR 遗传多样性 遗传结构 

分 类 号：S718.54[农业科学—林学] S718.46