卵巢癌中Rad51蛋白表达的意义  

Association between Rad51 protein expression and metastasis of ovarian cancer

作　　者：闫芸芳[1] 龙腾飞[1] 周家德[1] 

机构地区：[1]安徽医科大学第一附属医院妇产科,合肥230022

出　　处：《安徽医学》2014年第1期23-26,共4页Anhui Medical Journal

摘　　要：目的探讨DNA双链修复蛋白(Rad51蛋白)在不同病理分期及组织分型的卵巢癌组织中的表达水平,及其与卵巢癌发生、发展、侵袭及转移的相关性。方法应用免疫组织化学染色法(immunohistochemistry,IHC)EnVisionTM二步法检测62例人卵巢癌组织及10例卵巢良性肿瘤组织中Rad51蛋白的表达。结果卵巢癌组织中Rad51表达率明显高于卵巢良性肿瘤组织(P<0.05);Rad51蛋白与卵巢癌临床分期有关(P<0.05),随肿瘤恶性程度的增高而上升;Rad51蛋白表达与卵巢癌组织分型有关(P<0.05),Rad51蛋白在上皮性卵巢癌(如黏液性囊性癌、浆液性囊腺癌、子宫内膜样腺癌、透明细胞癌)较非上皮性卵巢癌(如无性细胞瘤、内胚窦瘤、未成熟畸胎瘤等)表达明显增高(P<0.05)。结论 Rad51可能参与卵巢癌的发生发展,并与组织类型相关。Objective To investigate the expression of the repair of DNA double-strand protein （ Rad51 protein） in different patho- logical stages and histological type of ovarian cancer, as well as the relationship with the occurrence, development, invasion and metastasis of ovarian cancer. Methods The expression of Rad51 was detected in 62 cases of ovarian carcinoma and 10 cases of benign ovarian tumor tis- sues without cancer by using immunohistochemical staining （ Immunohistoehemistry, IHC ）. Results The expression of Rad51 in ovarian car- cinoma tissues was significantly higher than that of ovarian non-cancerous tissues （ P 〈 0.05 ）. The expression of RadS1 protein was correlated with clinical stages （P 〈 0.05 ） , increased with the increased malignancy of the tumor. The expression of Rad51 protein was also related to different types of ovarian cancer, which was significantly higher in epithelial ovarian cancer （ such as mucinous cystic carcinoma, serous, endo- metrioid carcinoma, and uterine clear cell carcinoma） than in the non-epithelial ovarian cancer （such as dysgerminoma, endodermal sinus tumor, immature teratoma and so on） （P 〈 0.05 ）. No significant correlation was found between the expression of Rad51 and the age, metasta- sis and histological grade. Conclusion The expression of Rad51 protein is correlated with tumor metastasis in ovarian cancer, and the occur- rence may play a role in the formation and development in ovarian cancer.

关 键 词：Rad51蛋白 卵巢癌 同源重组 

分 类 号：R737.31[医药卫生—肿瘤]