亲环素基因GhCYP1在陆地棉中的过量表达及耐盐性分析  

Overexpression of Cyclophilin Gene(GhCYP1)in Trangentic Cotton and Its Salt Tolerance Analysis

作　　者：张安红[1] 王志安[1] 肖娟丽[1] 罗晓丽[1] 

机构地区：[1]山西省农业科学院棉花研究所,山西运城044000

出　　处：《山西农业科学》2014年第2期107-109,122,共4页Journal of Shanxi Agricultural Sciences

基　　金：山西省青年科技研究基金项目(2012021023-2)

摘　　要：通过农杆菌介导法将亲环素基因GhCYP1导入陆地棉棉花栽培品种中棉35中,经过卡那霉素抗性选择、PCR检测和系统选育获得5个不同转基因纯合系.在温室盆栽条件下,于3片真叶期对转基因棉花纯合系和非转基因对照进行200 mmol/L NaCl胁迫处理20d.结果表明,转GhCYP1基因棉花株系比对照长势强,株高比对照提高2～5cm,地上部分单株鲜质量比对照增加7.1％～12.4％,抗氧化物酶SOD,POD,CAT等的活性以及叶绿素含量显著高于对照.说明过量表达GhCYP1基因提高了陆地棉对盐碱的抗性.Transgenic plants of cotton cultivar Zhongmian 35 were obtained by Agro-bacterium-mediated transformation of the ex- pression GhCYP1 gene, Kanamycin, PCR, and system seletion analyses showed that the GhCYP1 gene was integrated into cotton genome, it obtained 5 different trangentic homozygous lines. Under greenhouse conditions, 200 mmol/L NaC1 was applied for 20 d. The result showed： the transgenic cotton plants grew normally under NaC1 stresses with increase of 2-5 cm for plant height and 7.1%-12.4% for fresh weight plant compared with the control plants; the content of chlorophy in the transgentic lines were higher than those of non- transgentic plants. In addition, the activities of antioxidant enzymes, such as SOD, CAT, POD were higher than those of non-transgentic cottons. These studies demonstrated that the GhCYP1 gene could overexpress in the cotton, which could be used in genetic engineering for cotton salinity resistance.Transgenic plants of cotton cultivar Zhongmian 35 were obtained by Agro-bacterium-mediated transformation of the ex- pression GhCYP1 gene, Kanamycin, PCR, and system seletion analyses showed that the GhCYP1 gene was integrated into cotton genome, it obtained 5 different trangentic homozygous lines. Under greenhouse conditions, 200 mmol/L NaC1 was applied for 20 d. The result showed： the transgenic cotton plants grew normally under NaC1 stresses with increase of 2-5 cm for plant height and 7.1%-12.4% for fresh weight plant compared with the control plants; the content of chlorophy in the transgentic lines were higher than those of non- transgentic plants. In addition, the activities of antioxidant enzymes, such as SOD, CAT, POD were higher than those of non-transgentic cottons. These studies demonstrated that the GhCYP1 gene could overexpress in the cotton, which could be used in genetic engineering for cotton salinity resistance.

关 键 词：棉花 亲环素基因 耐盐 转基因 CYCLOPHILIN GENE (GhCYP1) 

分 类 号：S562[农业科学—作物学]