不同下颌前伸力对髁突软骨细胞中Sox9和Ⅱ型胶原表达的影响及其意义  

Effects of different modes of mandibular advancement on expressions of Sox9and collagenⅡin condylar chondrocytes and their significances

作　　者：刘莹[1] 刘艳艳[2] 王硕[1] 袁洋[1] 刘畅[1] 

机构地区：[1]吉林大学口腔医院正畸科,吉林长春130021 [2]吉林大学南岭校区医院口腔科,吉林长春130022

出　　处：《吉林大学学报（医学版）》2014年第1期97-101,I0003,共6页Journal of Jilin University：Medicine Edition

基　　金：吉林省卫生厅科研基金资助课题(2008Z010);吉林大学基本科研业务费-科学前沿与交叉学科创新项目资助课题(200903225)

摘　　要：目的:探讨施加不同作用方式的下颌前伸力后大鼠髁突软骨细胞中Sox9及Ⅱ型胶原表达水平,阐明不同作用方式的下颌前伸力对其表达及软骨形成的影响。方法:将28只5周龄雄性Wistar大鼠按下颌前伸力的不同作用方式随机分为动态组、静态组、功能组和对照组(n=7)。各组大鼠按照设定的相关参数接受不同的下颌前伸力刺激。2周后实验结束时取颞下颌关节标本,行免疫组织化学染色,对髁突软骨细胞中Sox9和Ⅱ型胶原的表达进行半定量分析。结果:对照组、动态组和功能组大鼠髁突软骨细胞中Sox9阳性表达细胞数显著高于静态组(P<0.01),动态组和对照组大鼠髁突Sox9阳性表达面积显著高于静态组和功能组(P<0.01),功能组Ⅱ型胶原阳性表达面积显著高于其他3组(P<0.01),静态组Ⅱ型胶原阳性表达面积显著高于对照组(P<0.05)。结论:下颌前伸力的作用方式及作用时间是调控髁突软骨细胞中Sox9及Ⅱ型胶原表达水平的关键因素。Objective To study the expression levels of Sox9 and collagen II in condylar chondrocytes after treated with different modes of mandibular advancement in rats, and to clarify the effects of different modes of mandibular advancement on the expressions of Sox9 and collagen I1 as well as formation of cartilage in condyle. Methods Twenty-eight male 35-day-old Wistar rats were randomly divided into dynamic, static, functional and control groups （n = 7）, and received different modes of mandibular advancement according to their groups. 2 weeks after the experiment, temporomandibular joint sections were prepared for immunohistochemical staining, and the expression levels of Sox9 and collagen II in condylar chondrocytes were semi-quantitatively evaluated. Results The number of Sox9 positive cells in control group, dynamic group and functional group was significantly higher than that in static group （P〈0.01）, the Sox9 positive expression areas in dynamic group and control groupwere statistically larger than those in static group and functional group （P〈0.01）, the positive expression area of collagen II in functional group was significantly higher than those in the other three groups （P〈0.01）, and the positive expression area of collagen ]1 in static group was higher than that in control group （P 〈0.05）. Conclusion The modes and duration of mandibular advancement play a key role in regulating the expressions of Sox9 and collagen II in condylar chondrocytes.

关 键 词：下颌前伸力 SOX9 Ⅱ型胶原 软骨内成骨 

分 类 号：R783[医药卫生—口腔医学]