Musashi1和Sox2蛋白在肺癌组织中的表达及其临床意义  

Expressions of Musashi1 and Sox2 proteins in lung cancer tissue and their clinical significances

作　　者：李劳冬[1] 莫碧文[1] 于会娜[2] 王昌明[1] 曾锦荣[1] 王绩英[1] 韦江红[1] 黄剑伟[1] 

机构地区：[1]桂林医学院附属医院呼吸内科,广西桂林541001 [2]湖北省荆州市中心医院儿科,湖北荆州434000

出　　处：《吉林大学学报（医学版）》2014年第1期141-145,I0004,共6页Journal of Jilin University：Medicine Edition

基　　金：广西壮族自治区卫生厅重点科研项目资助课题(重2012003)

摘　　要：目的:探讨干细胞标志物Musashi1和性别决定区Y框架蛋白2(Sox2)在肺癌组织中的表达情况,阐明二者在肺癌发生发展过程中的作用及其临床意义。方法:采用免疫组织化学法检测50例肺癌组织、32良性病变肺组织和18例癌旁正常肺组织中Musashi1和Sox2蛋白的表达水平,并分析二者的表达水平与肺癌临床病理参数的关系。结果:Musashi1在肺癌组织、良性病变肺组织和癌旁正常肺组织中的阳性表达率分别为50%(25/50)、0(0/32)和0(0/18),在肺癌组织中的阳性表达率显著高于良性病变肺组织和癌旁正常肺组织(P<0.001);Musashi1表达强度与肺癌的组织学类型和分化程度有关联(P<0.05),与年龄、性别、TNM分期和淋巴结转移无关(P>0.05)。Sox2在肺癌组织、良性病变肺组织和癌旁正常肺组织中的中-强度阳性表达率分别为64%(32/50)、9.4%(3/32)和0(0/18),在肺癌组织中的中-强度阳性表达率显著高于良性病变肺组织和癌旁正常肺组织(P<0.001);Sox2的表达强度与肺癌的组织类型有关联(P<0.001),与年龄、性别、TNM分期、淋巴结转移和分化程度无关(P>0.05)。肺癌组织中Musashi1和Sox2的阳性表达强度呈正相关关系(r=0.598,P<0.001)。结论:Musashi1和Sox2蛋白在肺癌组织中表达上调,提示二者在肺癌的发生发展过程中可能起重要作用,有望成为新的肺癌诊断标志物和治疗靶点。Objective To study the expressions of Musashil and sex determining region Y-box 2 （Sox2） proteins in lung cancer, benign lesion lung and normal cancer-adjacent lung tissues, and to explore the clinical significances of Musashil and Sox2 proteins in the carcinogenesis and progression of lung cancer. Methods The expressions of Musashil and SoX2 proteins＇ were detected by immunohistochemistry in 50 cases of lung cancer tissue, 32 cases of benign lesion lung tissu：e and 18 cases of normal cancer-adjacent lung tissue. The relationships between the expressions of Musashil and Sox2 proteins and clinical pathological parameters were analyzed. Results The positive rates of Musashil expression in lung cancer tissue, benign lesion lung tissue and normal cancer-adjacent lung tissue were 50% （25/50）, 0 （0/32）, and 0 （0/18）. The expression rate of Musashil in lung cancer tissue was significantly higher than those in benign lesion lung tissue and normal cancer-adjacent lung tissue （P〈0. 001）. The expression of Musashil was significantly correlated with the histological types and differentiation of lung cancer （P〈0.05）, but was not correlated with the age, gender, TNM stage and lymph node metastasis （P〉0.05）. The positive rates of Sox2 moderate to strong expression in lung cancer tissue, benign lesion lung tissue and normal cancer-adjacent lung tissue were 64% （32/50）, 9.4% （3/32）, and 0 （0/18） . The expression rate of Sox2 in lung cancer tissue was significantly higher than those in benign lesion lung tissue and normal cancer-adjacent lung tissue （P〈0. 001）. The expression of Sox2 was significantly correlated with the histological type of lung cancer （P〈 0. 001）, but was not correlated with the age, gender, TNM stage, lymph node metastasis and differentiation （P〉0.05）. The expression of Musashil in lung cancer tissue was positively correlated with the expression of Sox2 （r=0. 598, P〈0. 001）. Conclusion The up-regulation of Musashil and Sox2 in lung cancer

关 键 词：肺肿瘤 Musashi1 性别决定区Y框架蛋白2 干细胞标志物 诊断标志物 治疗靶点 

分 类 号：R734.2[医药卫生—肿瘤]