生物表面活性剂鼠李糖脂对白腐真菌降解DDTs的影响  

Effects of Biosurfactant Rhamnolipid on Biodegradation of DDTs by White Rot Fungus

作　　者：肖鹏飞[1] 尤铁学[1] 宋玉珍[1] 王剑桥[2] 

机构地区：[1]东北林业大学林学院,黑龙江哈尔滨150040 [2]静冈大学农学院

出　　处：《环境科学与技术》2014年第2期29-33,共5页Environmental Science & Technology

基　　金：黑龙江省教育厅科学技术研究项目资助(12533010)

摘　　要：研究了生物表面活性剂鼠李糖脂对DDTs在水相中的增溶作用，对白腐真菌PhlebialindtneriGBl027生长的影响以及对该菌株降解DDTs的影响。结果表明，高于临界胶束浓度（cMc）的鼠李糖脂可明显增加各DDTs的表观溶解度，其中对4，4’一DDD的增溶效果最好。低浓度（〈O．05g／L）的鼠李糖脂会促进白腐真菌在PDB培养基中的生长，而高于0．1g／L浓度的鼠李糖脂则对菌株的生长产生一定的抑制作用，最高抑制率可达到42％。在0．02～0．5g／L浓度范围内，鼠李糖脂可促进白腐真菌对DDTs的降解效果，且降解率随着鼠李糖脂浓度的增加而增加。在0．5g／L的鼠李糖脂添加体系培养14d后，白腐真菌对4，4＇-DDT、2，4’-DDT、4，4’-DDD和2，4’一DDD的降解率分别提高了24．0％，22．8％，25．06％和22．0％。而鼠李糖脂浓度进一步升高至1．0g／L时，由于菌株的生长受到抑制从而导致对DDTs降解率的下降。Effects of biosurfactant rhamnolipid on solubilization of DDTs, growth of white rot fungus Phlebia lindtneri GB1027,and fungal biodegradation of DDTs were investigated. Results showed that the concentrations of rhamnolipid above the critical micelle concentration （CMC） could increase the solubility of DDTs to great extent. Rhamnolipid with low concentrations （〈0.05 g/L） had positive effects on fungal growth in PDB medium, whereas rhamnolipid of high concentration （ 1.0 g/L） inhibited the fungal growth rate by 42.0%. Rhamnolipid could enhance the degradation of DDTs at a concentration range between 0.02 and 0.5 g/L by P. lindtneri GB1027,and the degradation rate increased with increasing surfactant concentration. The highest efficiencies were increased by 24.0%, 22.8%, 25.0% and 22.0% for 4,4＇ -DDT, 2,4＇ -DDT, 4,4＇ - DDD and 2,4＇-DDD within 14 days respectively, compared to the control culture without surfactant, when the concentration of rhamnolipid was 0.5 g/L. However 1.0 g/L of rhamnolipid reduced the degradation of DDTs because of its toxicity to P. lindtneri GB1027.

关 键 词：生物表面活性剂 鼠李糖脂 白腐真菌 生物降解 DDTS 

分 类 号：X172[环境科学与工程—环境科学]