同步衍生化法测定龙葵中还原型谷胱甘肽(GSH)和总巯基(—SH)含量  

Determination of Reduced Glutathione( GSH) and Total Thiols( —SH) in Solanum Nigrmn L. by in Situ Derivatization Methods

作　　者：张小勇[1] 朴玉莲[1] 崔胜云[1] 

机构地区：[1]延边大学长白山生物资源与功能分子教育部重点实验室,吉林延吉133002

出　　处：《化学试剂》2014年第2期147-149,172,共4页Chemical Reagents

基　　金：国家自然科学基金资助项目(21165021)

摘　　要：用衍生化试剂邻苯二甲醛(OPA)和4,4'-二硫代联吡啶(4-DPS),采用细胞破壁、衍生化为一体的同步提取方法,分别采用荧光光度法和UV-Vis分光光度法测定了龙葵鲜叶中还原型谷胱甘肽(GSH)和总巯基(—SH)含量。龙葵鲜叶GSH和总巯基(—SH)含量分别为0.115 1和0.387 8μmol/g,方法的回收率分别达101.78%和98.36%,检测限分别为0.154 7和0.899 4μmol/L。该方法灵敏、可靠且满足植物鲜品巯基化合物的灵敏测定。Reduced glutathione and total thiols have been de- termined in the fresh leaves of Solanum nigrmn L by in situ derivatization during ultrasonic cell rupture using o-phthalde- hyde （ OPA ） and 4,4＇-dithiodipyridine （4-DPS） as derivat- izing agents by fluorophotometric and UV-Vis spectrophoto- metric methods. The amounts of GSH and total thiols （-SH） determined in the fresh leaves of Solanum nigrmn L are 0. 115 1μmoL/g and 0. 387 8μmoL/g respectively. The recoveries of the methods are 101.78% and 98.36% , detection limits are 0. 154 7 μmol/L and 0. 899 g μmol/L respectively. The methods are sensitive and accurate for the determination of thiol compounds in fresh plant samples.

关 键 词：龙葵 谷胱甘肽 巯基化合物 邻苯二甲醛 4 4’-二硫代联吡啶 

分 类 号：O657.39[理学—分析化学] O657.32[理学—化学]