DNMT1和HDAC1在上皮性卵巢癌组织中的表达及临床意义  

DNMT1 and HDAC1 expression in epithelial ovarian cancer tissue and its clinical significance

作　　者：王艳云[1] 杨秋丽[2] 刘丽霞[3] 何秀萍[1] 

机构地区：[1]哈尔滨医科大学附属第一临床医学院妇产科,黑龙江哈尔滨150000 [2]内蒙古医科大学第三附属医院妇产科,内蒙古包头014010 [3]牡丹江医学院红旗医院妇产科,黑龙江牡丹江157011

出　　处：《国际检验医学杂志》2014年第3期277-279,共3页International Journal of Laboratory Medicine

摘　　要：目的研究DNA甲基转移酶(DNMT)1和组蛋白去乙酰化酶(HDAC)1在正常卵巢组织、良性卵巢肿瘤组织及上皮性卵巢癌组织中的表达,分析二者与卵巢癌组织多种临床病理参数关系及相关性。方法运用免疫组织化学方法检测15例正常卵巢组织、20例良性卵巢肿瘤组织及30例上皮性卵巢癌组织中DNMT1和HDAC1的表达情况。分析二者的表达与卵巢癌临床病理参数的关系,以及2种蛋白在卵巢癌组织中表达的相关性。结果上皮性卵巢癌组织中DNMT1和HDAC1的表达高于正常卵巢组织和良性卵巢肿瘤组织,差异有统计学意义(P<0.05);卵巢癌组织中DNMT1和HDAC1的表达与是否绝经和病理类型无关,而与组织学分级、临床分期有关;DNMT1和HDAC1二者在卵巢癌组织中的表达呈正相关。结论 DNMT1和HDAC1的高表达在卵巢癌发生、发展中有重要作用。二者具有协同作用是卵巢癌发生发展的主要原因,可以为卵巢癌的早期筛查提供理论依据。Objective To investigate the expression of DNMT1 and HI）AC1 in normal ovarian tissue,benign ovarian tumor tis- sue and epithelial ovarian cancer tissue. Methods Immunohistochemical method was used to detect the DNMT1 and HDACl ex pressions in 15 normal ovarian tissues, 20 benign ovarian tumor, and 30 epithelial ovarian cancer tissue. The relationship between ex pression and clinic pathological parameters of ovarian cancer were analyzed. Results DNMT1 and HDAC1 positive expression in epithelial ovarian cancer was significantly higher than that of normal ovarian tissue and benign ovarian tumor tissues （P〉0.05）. In ovarian cancer tissue,DNMT1 and HDAC1 expressions had no significant relationship with the presence of menopause, pathological types,and histological grade. DNMT1 and HDAC1 expression in ovarian cancer tissue were positively correlated. Conclusion High expression of DNMT1 and HI）AC1 plays an important role in the occurrence and development of ovarian cancer.

关 键 词：甲基转移酶类 组蛋白去乙酰化酶 免疫组织化学 卵巢肿瘤 

分 类 号：R737.31[医药卫生—肿瘤]