外周血游离LUNX mRNA检测在非小细胞肺癌患者辅助诊断中的意义  

Clinical significance of cell-free LUNX mRNA detection in peripheral blood in patients with non-small cell lung cancer

作　　者：柳一花[1] 周洪兴[1] 张平[1] 孙颖昕 王文伟[1] 白阳[1] 周韩[1] 陈磊[1] 

机构地区：[1]南京医科大学附属常州市第二人民医院检验科,江苏常州213003

出　　处：《国际检验医学杂志》2014年第3期284-285,289,共3页International Journal of Laboratory Medicine

摘　　要：目的检测非小细胞肺癌(NSCLC)患者外周血细胞外游离LUNXmRNA,探讨其在NSCLC辅助诊断中的意义。方法选择原发性NSCLC患者65例,将体检正常的50例健康者设为对照组。采用实时聚合酶链反应(RT-PCR)检测其外周血游离LUNXmRNA,评价该指标对NSCLC辅助诊断的价值。结果 65例肺癌患者38例外周血游离LUNXmRNA检出阳性,阳性率显著高于健康体检者,差异有统计学意义(P<0.01);低分化以及晚期肺癌患者该基因检出阳性率高于中高分化及早期肺癌患者,差异有统计学意义(P<0.01);LUNXmRNA阳性率与患者性别、年龄、肿瘤组织类型、发生部位及患者吸烟与否无关。结论外周血游离LUNXmRNA检测对NSCLC具有特异辅助诊断价值。Objective To investigate the clinical significance of cell-free LUNX mRNA in peripheral blood in patients with non small cell lung cancer. Methods Cell free LUNX mRNA was detected by real-time PCR in 65 patients with non-small cell lung cancer and 50 healthy subjects. Results The positive LUNX mRNA rate was 58.5% （38 in 65） in lung cancer patients,which was significantly higher than in healthy subjects （P〈0.01）. The positive rates in poorly differentiated and advanced stage cancer pa- tients were significantly higher than that in lung cancer patients with high differentiation and early stage （P〈0.01）. Positive LUNX mRNA had no correlation with gender,age,histological type,tumor location and smoking. Conclusion Detection of cell free LUNX mRNA in peripheral blood can be used in auxiliary diagnosis of non-small cell lung cancer with high specificity.

关 键 词：癌 非小细胞肺 聚合酶链反应 诊断 

分 类 号：R734.2[医药卫生—肿瘤]